Journal of Experimental Pathology
ISSN: 2694-5061

Review Article - Journal of Experimental Pathology (2021) Volume 2, Issue 2

Faecal Microbiota Transplantation as Primary Treatment for Clostridioidoes difficile Infection-evidence for Change

Sibasish Dolai1,2*, John Ng2, Sabine Hazan3, Vic Dawson2, Thomas J Borody1*

1The Centre for Digestive Diseases, 229 Great North Road, Five Dock, Sydney, Australia

2Axent Medical Pty Ltd, CSIRO Building 53, 11 Julius Avenue, North Ryde, Sydney, Australia

3Progenabiome, 1845 Knoll Dr, Ventura, CA 93003, USA

*Corresponding Author:
Sibasish Dolai
E-mail:Siba.Dolai@cdd.com.au
 
Thomas J Borody
E-mail:Thomas.Borody@cdd.com.au

Received date: May 15, 2021; Accepted date: May 27, 2021

Citation: Dolai S, Ng J, Hazan S, Dawson V, Borody TJ. Faecal Microbiota Transplantation as Primary Treatment for Clostridioidoes difficile Infection-evidence for Change. J Exp Pathol. 2021;2(2):90-101.

Copyright: © 2021 Dolai S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Abstract

In this mini-review, we summarise the significant body of evidence for the treatment of Clostridioides difficile infection (CDI) with Faecal Microbiota Transplantation (FMT) and propose the transition of FMT from ‘last resort’ treatment to the forefront of CDI management. To address the feasibility of this proposal, we examined the rates of efficacy in FMT treated patients with CDI and also reviewed the safety of FMT across available published studies. A considered view of safety, efficacy, product standardisation, quality control and Good Manufacturing Practices (GMP) allows for a prudent approach in positioning FMT as the primary or initial treatment for CDI.


Keywords

Faecal microbiota transplantation, Clostridioides difficile infection, Recurrent, Treatment, Safety, Efficacy, Donor screening, Colonoscopy, Nasogastric, Review


Introduction

Clostridioides difficile is an anaerobic spore-forming gram positive bacillus that infects the gut microbiota leading to gastrointestinal disease with varied severities ranging from self-limiting diarrhea to life-threatening pseudomembranous colitis, sepsis or death. In the last 20 years, there has been a marked increase in Clostridioides difficile infection (CDI) worldwide with almost 1% of all hospitalizations or 329,460 patients in the United States in 2017, which is due to CDI [1]. In part, this has been due to CDI also becoming more virulent, refractory, and relapsing after standard antibiotic therapy [2]. Current treatment guidelines specify antibiotics as frontline therapy (vancomycin, metronidazole, and fidaxomicin), however, such treatments may deplete bacterial classes and result in non-uniform intestinal microbiota dominated by Proteobacteria [3]. This preliminary antibiotic-induced dysbiosis may further promote resistance leading to relapsed or refractory CDI. Published data shows the relapse rate with vancomycin and metronidazole treatment to be as high as 37% and 50%, respectively [4]. On the other hand, there is now considerable evidence that (FMT) effectively cures CDI and may emerge as an effective initial treatment of CDI even in patients diagnosed with CDI for the first time [5]. There is now evidence to demonstrate a cure rate of up to 98% when more than one FMT infusion is performed [6,7]. Despite this growing new data, the FDA guidelines may be holding physicians back from curing more CDI patients. Current guidelines only approve a single FMT procedure and only in patients with two previous treatment failures with antibiotics [8,9].

Whilst FMT is becoming an increasingly common procedure, a minority of healthcare practitioners still hold reservations regarding its efficacy and safety. Primarily these perspectives have centred around safety, patient tolerability to colonoscopy and fear of infections from donor transplant material [10,11]. Indeed, while FMT has several contraindications [12] particularly for those with immunocompromised conditions, the risks of transmissible infection have been extremely low (as detailed later).

Since 2015 medical professionals have been debating positioning FMT as first-line therapy for CDI [13]. Given FMT involves the transplantation of a highly heterogeneous biological sample (stool) from a healthy donor to the sick patient, regulatory authorities are challenged with providing clear guidance for the regulation of the procedure. Currently, regulatory harmonization is lacking and guidelines continue to conservatively reserve FMT for unresponsive cases only [8]. More recently, FMT has also proved to be an effective treatment for a suite of other gastrointestinal and systemic diseases including Ulcerative Colitis (UC) and Irritable Bowel Syndrome (IBS) which has highlighted the need for further appraisal and guidelines on the standardisation of donor evaluations, production standards, and acceptable clinical indications. In 2019, published consensus statements from an international expert panel proposed a set of best practices in FMT [14], and in late 2020 the identification of possible COVID-19 transmission from FMT further highlighted the need for safety and certainty in donor screening [15].

The treatment of CDI is at a turning point. Given that: 1) FMT is very effective in curing CDI; 2) that using more than one FMT is more efficacious; 3) that antibiotics can compromise the microbiome and increase failure to cure (up to 60%), and 4) that FMT is a simple GI procedure best performed by trans-colonoscopic infusion or enema. Hence, we propose advancing FMT to a front line therapy to eradicate CDI. Furthermore, FMT has been demonstrated to repair the host’s microbiome from damage and replace missing flora simultaneously without delaying the recovery via using antibiotic pre-treatment. In this review, we also briefly update the recent data relating to FMT efficacy, safety and delivery methods in CDI management.


Evidence of Efficacy

Over several decades FMT has been trialled in various formats for CDI with very promising results. In a 2017 systematic review and meta-analysis Quraishi et al. assessed the results of thirty-seven studies including seven randomised controlled trials (RCTs) and 30 case series. FMT was found to be more effective than vancomycin in resolving recurrent and refractory CDI, and clinical resolution across all studies was 92%. Furthermore, administering consecutive courses of FMT following the failure of the first FMT resulted in incrementally improved effects. The review also showed that successful outcomes after FMT were independent of preparation and route of delivery [16]. Moayyedi et al. also released a smaller systematic review of FMT for CDI associated diarrhoea in the same year analysing ten RCTs that evaluated 657 patients. Within this dataset, five RCTs were identified that compared FMT against placebo or vancomycin (total of 284 patients) with FMT demonstrating strong statistical superiority [17]. FMT was also used in a proof-of-concept trial to evaluate its use as a treatment for primary CDI with 78% success compared to 45% in a control metronidazole group, again highlighting FMT as an alternative to antibiotic therapy in primary CDI [18]. The use of FMT as first-line treatment is further supported by a pooled analysis of FMT treatment outcomes between patients receiving vancomycin before treatment versus no pretreatment showing no difference in treatment outcomes [19].

The success of FMT by traditional colonoscopic infusion has also led to novel delivery systems to avoid the disadvantages of bowel prep, anaesthesia, and enteral access. Iqbal et al. performed a systematic review of FMT using donor samples in ingestible capsules. Covering a total of six studies, five case series and one randomized controlled trial, 341 patient data sets were analysed. Efficacy was observed in 285 patients in the first treatment, with no recurrence. An additional 28 patients received a second FMT with resolution and three received resolution on their third FMT. In sum, a resolution rate of 93%. Only one patient did not achieve long-term resolution of symptoms despite of receiving four treatments [20].

The majority of recorded studies have recruited patients diagnosed with recurrent symptoms of diarrhea and serially positive for C. difficile toxin on testing. In many cases, patients had been treated with several courses of Vancomycin with several failures to cure when tapering antibiotics. To investigate the effectiveness of FMT on their first episode of CDI diagnosis Roshan et al. completed a retrospective, single-centre study of 59 patients between 2012 and 2017 treated with FMT on their first episode of CDI [6]. In light of strong evidence that repeated FMT (more than one) leads to increased efficacy rates, all patients were first treated with colonoscopic infusion, followed by an enema the next day. 98% of the 58/59 patients were cleared of C. difficile as verified by culture and toxin testing over the next 4-8 weeks. There were also statistically significant symptomatic reductions in abdominal pain, diarrhoea, and blood in the stool [6].

The efficacy rates in treating CDI with FMT is summarized in (Table 1). Of note are the incremental increases in efficacy on second and third FMT infusions.

Year – Author1Method of delivery2Sample No.Efficacy After Single FMT (%)3Efficacy after additional FMTs (%)3
2020–Roshan et al. [6]Colonoscopy and enema5998
2020–Perler et al. [21]Colonscopy, capsule20785.595.1
2003–Aas et al. [22]Nasogastric1883.3
2016–Agrawal et al. [23]Esophagogastroduodenoscopy, push enteroscopy, colonoscopy, flexible sigmoidoscopy, and retention enema14682.995.9
2014–Allegretti et al. [24]Colonoscopy1384.692.3
2012–Brandt et al. [25]Colonoscopy779198
2014–Emanuelsson et al. [26]Rectal catheter3174
2015–Ganc et al. [27]Oral push enteroscopy1290
2010–Garborg et al. [28]Duodenal, colonoscopy297383
2012–Hamilton et al. [29]Colonoscopy438695
2016–Kelly et al. [30]Colonoscopy4690.993.5
2017–Kao et al. [31]Colonoscopy, capsule11696.2
2012–Kassam et al. [32]Enema278193
2012–Kelly et al. [33]Colonscopy2692
2014–Khan et al. [34]Colonoscopy2090100
2014–Lee et al. [35]Retention enema9447.986
2016–Lee et al. [36]Enema2198493
2009–Macconnachie et al. [37]Nasogastric157380
2011–Mattila et al. [38]Colonoscopy368994
2013–Patel et al. [39]Colonoscopy3190100
2013–Pathak et al. [40]Colonoscopy1292100
2014–Ray et al. [41]Colonoscopy20100
2010–Rohlke et al. [42]Colonoscopy1994100
2013–Rubin et al. [43]Nasogastric747990.7
2015–Tauxe et al. [44]Colonscopy and upper GI317587
2014–Vigvári et al. [45]Nasoduodenal15100
2010–Yoon et al. [46]Colonoscopy12100
2014–Zainah et al. [47]Nasogastric1479
2016–Allegretti et al. [24]Capsules1973.594
2015–Cammarota et al. [7]Colonoscopy206590
2015–Kao et al. [48]Colonoscopy, Capsule2996
2016–Lee et al. [36]Enema2325691
2013–Van Nood et al. [49]Duodenal infusion168194
2014–Youngster et al. [50]Capsule207090
2017–Hota et al. [51]Enema1643.8
2017–Jiang et al. [52]Colonoscopy7287
2017-Staley et al. [53]Capsule4988
2016–Youngster et al. [54]Capsule1808291
2018– Shogbesan et al. [55]Upper GI infusion, capsule in- gestion, colonoscopic infusion, or enema3038893

1Publications and authors are listed in detail in the references section. 2Descriptions of FMT delivery are provided in the latter part of this review. Where several methods are listed a review of several techniques or a comparison study may have been performed. Where applicable only the FMT treated arm was included. 3In some studies, a single primary FMT was performed without a secondary FMT, or vice versa. Where applicable these studies are denoted with (-).

Table 1: An efficacy summary of the literature tabulating treatment success rates after a single FMT procedure and after two or more procedures. Table includes both original studies and systematic analyses.

On comparing 25 of the studies in Table 1 that reported efficacy of single and multiple FMT’s, the mean efficacy of single FMT (80.17) versus >1 FMT (92.78) was a highly significant improvement, (p<0.00001) in achieving CDI cure. Please note some of these reports had overlapping data.


Evidence of Safety

FMT is generally considered safe with a very low risk of infection transmission. The majority of the shortterm safety concerns are attributable to the mode of administration rather than FMT itself. Several studies have been conducted recently to assess the efficacy and safety of FMT for the treatment of CDI. Saha et al. conducted a prospective survey-based study to assess the short term adverse events (n=609) and long term safety (n=447) of FMT for treating recurrent CDI (rCDI) [56]. The majority of the short term adverse events were limited to diarrhoea (>60%) and constipation (19%-33%) and the long term risk of adverse events and infectious complication were low [20]. Although several new diagnoses were observed in their study, none were related to FMT. Similarly, in the largest and longest study on efficacy and safety of FMT treatment of rCDI, Perler et al. reported long term cure with no adverse events related to FMT or downstream disease associated with dysbiosis [21]. Although safety concerns of post-FMT infection transmission was announced by the FDA, regarding 2 cases of drug resistant E. coli infections transmitted by donor stool, a growing number of studies have emphasized the rarity of infection transmission through FMT, even in high risk patient groups [8]. A review by Wang et al. reported remarkably rare infectious complications (2.5% of more than 1000 patients treated) post-FMT [57]. Low risks of FMT related infectious transmission was also observed in high-risk immunocompromised patients [55,58]. More recently, the FMT National registry, designed to assess FMT methods and both safety and effectiveness outcomes, also reported high effectiveness of FMT for CDI with a good safety profile [59]. Although the majority of FMT related adverse events and potential risk of infection transmission are mitigated by stringent selection and screening process of prospective donors, there are still a few reports of distinct adverse reactions.

Brumbaugh et al. performed an analysis in a mediumsized cohort of children where FMT was delivered by nasogastric feeding tube. A total of 47 procedures were performed in 42 children. Vomiting, which was selflimiting, was observed within 24 hours of the FMT occurring in 6 of 47 (13%) procedures [60]. Lai et al. conducted a larger study reviewing 407 articles that referenced FMT and their overall Adverse Events (AEs) [61]. In total 135 studies reported AEs for 4493 patients, with 36 studies experiencing no AEs. The commonest AEs in this group were related to the gastrointestinal system including diarrhoea (13.0%), abdominal distension/flatulence (11.6%), nausea/vomiting (6.1%), abdominal pain (5.5%) and constipation (2.1%). Other common AEs included fever (2.7%), respiratory difficulty (2.4%), headache (1.5%) and fatigue (1.4%). In IBD patients 1.3% experienced IBD flares or IBD-like symptoms. Despite several reviews reporting efficacy outcomes being independent of FMT type (i.e. oral vs colonoscopic), AEs and safety outcomes do appear to be somewhat related. The same review study found AE rates were generally higher in upper GI FMT compared to lower infusions, with exception to IBD cases where IBD flares and fevers were higher but not statistically significant (7.9% vs 1.7%) compared with those who had CDI only.

Soo et al. in 2020 similarly reviewed the short-term safety record of FMT finding very few adverse effects directly attributed to the procedure with most adverse events being self-limiting gastrointestinal symptoms including abdominal cramps, diarrhoea and constipation, which resolved within one week. Of note, the review reported two deaths from aspiration pneumonia related to sedation given at the time of faecal microbiota transplantation and one death from transmission of a multidrug resistant Escherichia coli [62,63]. Nasogastric and nasoduodenal delivery also tended to have higher rates of minor adverse effects relative to other methods despite the seemingly less-invasive procedure [64].

A comprehensive safety study into FMT was conducted by Cicilia Marcella et al. published in 2021. With a stricter set of inclusion criteria, 129 studies were included covering 4241 patients (5688 FMTs). AEs occurred in 19% of FMT procedures compared with <1% reported by Lai et al [61]. The most frequently reported AEs were diarrhoea (10%) and abdominal discomfort/pain/cramping (7%). FMTrelated serious adverse events (SAEs), including infections and deaths occured in 1.4% of patients who underwent FMT (0.99% microbiota-related SAEs). Four of five FMTrelated deaths were reported in patients receiving FMT via the upper gastrointestinal route. Of special note was that all reported FMT-related SAEs were in patients with mucosal barrier injury. The report however concluded that most FMT-related AEs were mild or moderate and self-limiting. Thus although FMT appears to be highly safe, AE’s were related to the method of implantation, thus highlighting the need for improvements to reduce both delivery-related AEs and, microbiota-related AEs [65].

There is a growing increase in the number of FMT indications such as inflammatory bowel disease (IBD), ulcerative colitis (UC) and Crohn’s disease which deserves mention. IBD /UC patients share some overlap with CDI with reported incidences ranging from 1.8%-4.6% [66]. There is some evidence that suggests FMT could worsen symptoms of underlying IBD. Qazi et al. reviewed twentynine studies including 514 IBD patients treated with FMT. The data showed a pooled rate of IBD worsening of 14.9%, with higher rates of IBD worsening using lower GI FMT (16.5%) compared with upper GI delivery (5.6%) [67]. Sood et al. analysed the data of 129 patients with active UC who received extensive multisession FMT via colonoscopy. Minor short-term adverse events included abdominal discomfort, flatulence, abdominal distension, borborygmi, and low-grade fever (30.8%, 15.9%, 9.8%, 7.9%, and 7.6%, respectively). Long-term adverse events included new-onset urticaria (4.3%), arthritis/arthralgia (6.5%), depression (2.2%), partial sensorineural hearing loss (2.2%), and allergic bronchitis (2.2%). Thirteen (12.9%) patients dropped out because of adverse events however it is unclear if these were linked to a single session FMT or the extended length and course of the trial [68]. Due to the prevalence of immunosuppressant medication in the above conditions, FMT studies in the immunosuppressed patient have also been investigated extensively. Shogbesan et al. reviewed forty-four studies covering 303 immunocompromised patients finding equivalent efficacy as high as 93% when multiple treatments were included. Serious AEs included 2 reported deaths, 2 colectomies, 5 treatment-related infections, and 10 subsequent hospitalizations. However, Shogbesan concluded these AE rates in immunocompromised patients were similar to those of immunocompetent patients [55].

In summary, FMT appears to be safe, as the majority of studies demonstrate that FMT doesn’t appear to raise adverse events above the rates seen in the underlying condition. However, in the case of colonoscopy based FMT it is difficult to ascertain whether adverse events (i.e. diarrhea, irregular bowel movements) are related to the FMT procedure or from underlying disease. In the rare reports of death following FMT, the elderly demographic and common presence of multiple co-morbidities makes it difficult to assess whether FMT has any interrelationship. Nevertheless, the nature of the procedure and the risks for donor-recipient infection, or immunological flare from IBD or other co-morbidity remains. Thus, vigilant implementation of standardised quality screening of both patient and recipient remains highly relevant.

Year – Author1Adverse Event Description2Cases ReviewedMinor Adverse Event No.2Serious Adverse Event No.2
2018–Iqbal et al. [20]Serious AE: high grade fever, Ulcerative Colitis341 3
2021–Saha et al. [56]Mild & Moderate AE: diarrhea (60%), constipation (60%), cramping (50%), IBS (16.9%) Unrelated Serious AE: unrelated deaths (3.8%)60936523
2016-Wang et al. [57]Mild & Moderate AE: all (28.5%) Serious AE: all (9.2%), death (3.5%), infection (2.5%), relapse of IBD (0.6%), CDI (0.9%)1089310100
2021–Kelly et al. [59]Mild & Moderate AE: diarrhea (2%), abdominal pain (2%) Serious AE: hospitalizations (1%), IBS (1%), IBD (1%)22297
2014–Youngster et al.  [50]Mild & Moderate AE: abdominal cramping and bloating3060
2016–Youngster et al.  [54]Mild & Moderate AE: fever, diarrhea, vomiting, nausea/ bloating, abdominal pain, fatigue, headache, other com- plaints (270 occurrences) Serious AE: fever, Ulcerative Colitis, hospitalised relapse (9), Unrelated Serious AE: hospitalisation, death (40)18011249
2018–Brumbaugh et al. [60]Mild & Moderate AE: vomiting4260
2019–Lai et al. [61]Mild & Moderate AE: diarrhoea (13.0%), abdominal distension/flatulence (11.6%), nausea/vomiting (6.1%), abdominal pain (5.5%) and constipation (2.1%), fever (2.7%), respiratory difficulty (2.4%), headache (1.5%) and fatigue (1.4%). 1.3% patients also experienced IBD flares or IBD-like symptoms. Serious AE: aspiration or aspiration pneumonia, sedation complications, bowel perforation, sepsis, hospitalisation, death.4609 21
2002–Cicilia Marcella et al. [65]Mild & Moderate AE: all (19%), diarrhoea (10%), abdominal discomfort/pain/cramping (7%). Serious AE: infections, death, other (1.4%).568887559
2018–Shogbesan et al.  [55]Mild & Moderate AE: abdominal pain, irritable bowel syndrome, nausea, fever, diverticulitis. Serious AE: colectomies, bacteremia, hospitalisation,in- flammatory bowel disease, pneumonia, aspiration pneu- monia, death.3032833
2013–Van Nood et al.  [49]Mild & Moderate AE: diarrhea (94%), cramping (31%),belching (19%), constipation (19%).16150
2016–Lee et al. [69]Mild & Moderate AE: transient diarrhea (70%), abdomi- nal cramps (10%), nausea (<5%), constipation (20%) and excess flatulence (25%) Serious AE: urinary tract infections (<5%), respiratory tract infection23216211
2015-Cammarota et al. [7]Mild & Moderate AE: diarrhoea (94%), bloating and ab- dominal cramping(60%)20190
2014–Dutta et al. [70]Mild & Moderate AE: low-grade fever (18%), bloating (11%).2780
2017–Jiang et al. [52]Mild & Moderate AE: nausea, mild diarrhoea and tran- sient abdominal discomfort (86%), fever(2%), fatigue (8%), headache (6%), weight gain (3%)72620
2016 Baxter et al. [71]Mild & Moderate AE: abdominal distension/bloating/ cramping (2.3%), flatulence (2%), diarrhoea (1.9%), irregular bowel movement (1.2%), IBS Symptoms (1.09%), constipation (1%), other (4%). Serious AE: bacteremia, perforations, death (3)11901623

1Publications and authors are listed in detail in the references section. 2Adverse events were categorised into mild & moderate and serious. Symptoms such as cramping or diarrhea may include overlap in the single participant. Percentage figures were provided in the description where available from original authors. In some studies, minor events were omitted and denoted with (-).

Table 2: A safety summary of the literature tabulating reported adverse events including both original studies and systematic analyses.


Protocols and Methods

Implementing fundamental safety precautions will limit the spread of pathogenic organisms to the recipient, thus selective and regular screening of donors has been the focus of most institutions performing FMT on a regular basis. Donor screening requirements include general health, absence of gastrointestinal (GI) disease and no antibiotic therapy in the last 90 days. Other exclusions include autoimmune disease, chronic pain syndromes, neurologic disease, certain neuropsychiatric syndromes, metabolic syndrome, obesity, moderate or severe malnutrition, malignancy, ongoing oncologic therapy and being immunocompromised, as well negative serological and stool test results (Table 3).

SerologicalStool
Human Immunodeficiency Virus (HIV)Hepatitis C (HBC IgM)Epstein-Barr virus (EBV)Clostridium difficile (EIA)
Treponema pallidumSyphilisHepatitis B (HBsAg)Clostridium difficile toxin PCR
Cytomegalovirus (CMV)Liver enzyme (Aminotransferases)H.pylori (EIA)Shigatoxin-producing Escherichia coli (STEC)
Hepatitis A (HAV IgM)Human T-Lymphotrophic virus (HTLV 1 & 2)Viral tests (Adenovirus, Norovirus, Rotavirus)Extended-spectrum beta- lactamase (ESBL)–producing Escherichia coli
Epstein-Barr virus (EBV)Hepatitis B (HBV IgM)Enteric pathogen culture (Salmonella, Shigella, Campylobacter)Parasitic tests, (Ovum, Isospora, Cryptosporidium, Giardia)

Table 3: A typical serological and stool screening list for donors [72,73].

As screening can be both time and resource intensive, to limit the delays in recruiting fresh donors, access to universal donors via a biobank allows physicians in the US to obtain stool from biobanks without needing to submit Investigational New Drug applications and more recently to be compliant with COVID safety precautions [74]. There are several notable stool biobanks globally. Terveer et al. compiled a brief list of faeces banks that have been well established and describes the methodologies used at the Netherlands Donor Feces Bank including detailed screening procedures, example questionnaires and personal interviews of the donors concerning risk factors for transmissible diseases [75]. Such risk factors for transmissible disease include body mass index (BMI) > 25 kg/m2; as obesity may also be associated with a specific microbiota composition and other gastrointestinal disorder (e.g., irritable bowel syndrome (IBS), Crohn’s disease, or ulcerative colitis) which are increasingly showing a link to the host’s microbiome. These exclusion criteria, taken altogether, limit the risks of biota-based infection risks to the recipient which has been the main attention of safety concerns. Biobanking also provides the ability to track, measure and validate success rates from specific donor batches which provides added safety precautions, eliminating the risk factors associated from new donors in fresh transplant procedures.

Next, the physician has several options in the choice of transplantation into the GI tract. FMT has been shown to be robust and viably delivered to the lower gut through specialised tools such as colonoscopy, ileocolonoscopy, enema, distal ileum stoma and colostomy. Historically, the most validated procedure has been through retention enema or infusions into the colon aided by colonoscopy [76]. Where patients are deemed unsuitable for a colonic based infusion, methods for upper and mid-gut delivery (stomach, duodenum) have also been well developed; for example, FMT via gastroscope and the nasogastric tube [77]. This method involves insertion of a tube via the nasal cavity, or orally to reach the intestinal tract and the use of smaller more concentrated infusions of 30mL to 60mL with or without x-ray guidance and or the use of a gastroscope [77]. More recently, a few groups have concentrated donor stool into orally ingested capsules which has avoided the need for anesthesia and enteral access altogether, with some reports of equivalent efficacy rates comparable to colonoscopic procedures [31,54,78]. Though this approach appears promising, laboratory facilities to centrifuge and concentrate faecal samples are required, and the ingestion of 30-40 capsules can be challenging in some patients. Nevertheless, further randomised clinical trials are needed to validate this recent approach.


Conclusions

FMT is a proven effective treatment for rCDI. In this review, we highlighted the effectiveness and safety of FMT in CDI and thus demonstrated its readiness as the initial treatment for CDI. While this review only briefly covers issues of efficacy, safety and delivery, we believe that in general cases of CDI there is strong evidence to remove antibiotic treatment which is known to delay cure, promote antimicrobial resistance, dysbiosis and induce CDI recurrence when treatment is tapered. This study was limited by the availability of primary treatment data whereby patients are treated with FMT without antibiotic treatment. Ultimately, larger datasets of primary treatment outcome studies will be the greatest asset in assessing FMT for widespread application. Although the majority of FMT is well tolerated with only minor adverse events and rare infection transmission, there have been some notable adverse events. To advance FMT to the first line of CDI treatment safely, uniform and stringent regulatory guidelines need to be implemented regarding the source, production and processing of the transplantable FMT products. Recently, GMP manufacturing guidelines for FMT products have been established in some countries which have led to improved availability, safety, standardisation, and quality control of FMT products. Indeed, implementing widespread quality control of GMP–FMT products could raise the effectiveness and safety standards for FMT in line with other biological therapies. Whilst there remain questions on the underlying mechanisms of action and cure, or how FMT re-establishes microbial diversity, we propose that FMT should now become the first-line treatment for CDI.


Conflicts of Interest

SD, JN, SH, VD- none.

TJB is the Medical Director of Centre for Digestive Diseases offering FMT, and has filed patents in this field.


Author Contributions Statement

T.B. and S.D. conceived of the presented idea. S.D., J.N, S.H developed the review structure. V.D. encouraged and supervised J.N. to investigate and further research the findings presented in this work.


Funding Statement

None.


References