Abstract
Introduction: Inflammation is a natural defense response of higher organisms to various external aggressors, such as physicochemical factors or microbial infections. The objective of this work is to enhance a medicinal formula derived from a mixture of Gossypium hirsutum L. and Terminalia catappa L. by evaluating and improving its anti-inflammatory activity.
Methods: The two plant species selected for this study are Gossypium hirsutum L. and Terminalia catappa L. The aqueous extract of the medicinal formulation was obtained by mixing 42 g of Gossypium hirsutum L. powder with 24 g of Terminalia catappa L. pericarp powder, then 4.5 L of distilled water was added to this mixture and boiled for 30 min. The anti-inflammatory activity in rats was evaluated using the 1% carrageenan edema induction method, which triggers an inflammatory response. Calipers were used to assess the size of the edema in rats. The C-reactive protein (CRP) assay was performed using a COBAS 311 (Roche Hitachi) analyzer.
Results: The percentage of inhibition of AEGhTc 300 mg/kg of pw was 42.50 ± 05.70 % after 3 hours compared to 26.73± 10.00% for diclofenac at 10 mg/kg (P<0.05). It is important to note that the 300 mg/kg dose of AEGhTc (0.03 ± 0.02 mg/l) induced the lowest CRP concentration compared to the highest doses of AEGhTc 1000 and AEGhTc 2000 (0.28 ± 0.15 mg/l and 0.07 ± 0.04 mg/l).
Conclusion: The aqueous extract of the mixture of Gossypium hirsutum L. and Terminalia catappa L. exhibited significant anti-inflammatory activity at a dose of 300 mg/kg of AEGhTc.
Keywords
Gossypium hirsutum L, Terminalia cattapa L, Aqueous extract, Inflammation