Research Article - Journal of Cellular Immunology (2022) Volume 4, Issue 1
Comparison of Gene Editing versus a Neutrophil Elastase Inhibitor as Potential Therapies for ELANE Neutropenia
Vahagn Makaryan, Merideth Kelley, Breanna Fletcher, Isabella Archibald, Tanoya Poulsen, David Dale*
University of Washington, Department of Medicine, Seattle, Washington, USA
- *Corresponding Author:
- David C. Dale, MD,
Received date: December 07, 2021; Accepted date: January 28, 2022
Citation: Makaryan V, Kelley M, Fletcher B, Archibald I, Poulsen T, Dale D. Comparison of Gene Editing versus a Neutrophil
Elastase Inhibitor as Potential Therapies for ELANE Neutropenia. J Cell Immunol. 2022;4(1):19-28.
Copyright: © 2022 Makaryan V, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Heterozygous mutations in ELANE, the gene for neutrophil elastase, cause cyclic and congenital neutropenia through the programed cell death of neutrophil progenitors in the bone marrow. Granulocyte colony-stimulating factor is an effective therapy for these diseases, but alternative therapies are needed, especially for patients who do not respond well or are at high risk of developing myeloid malignancies. We developed an HL60 cell model for ELANE neutropenia and previously demonstrated that transient and regulated expression of mutant ELANE causes cell death by accelerated apoptosis. Knocking down the mutant gene or exposure to a potent inhibitor of neutrophil elastase rescued neutrophil development. Because of the great diversity in causative ELANE mutations, we generated stable HL60 clones expressing mutant P139L, C151Y and G214R and compared the effects of elastase inhibitor exposure to an ELANE knock-out line on cell development and function. ATRA induced differentiation demonstrated comparably impaired myeloid cell development for all three lines with upregulated expression of GRP78/BIP, an abnormality corrected by exposure of these cells to the elastase inhibitor MK-0339. The inhibitor and KO of mutant ELANE led to formation of neutrophils with comparable chemotactic and bactericidal capacities. We concluded that both strategies have great potential for the treatment of cyclic and congenital neutropenia. However, an orally absorbed, cell permeable inhibitor of neutrophil elastase, if proven safe and effective in a clinical trial, might be the better alternative to G-CSF or gene editing to treat ELANE neutropenia.
CRISPR/Cas9, G-CSF, Myeloid, ELANE KO, Neutrophil elastase, Inhibitors, Neutropenia
Mutations in ELANE, the gene for neutrophil elastase (NE), are the most common cause of cyclic and severe congenital neutropenia. These are very serious diseases, predisposing patients to severe bacterial infections. Both conditions are treated effectively now with recombinant human granulocyte colony-stimulating factor (G-CSF), but this treatment requires daily or alternate day subcutaneous injections and carries the potential risk of stimulating development of myeloid leukemia. Currently hematopoietic stem cell transplantation is the only other effective treatment; thus, there is a need for alternative therapies [1-11].
HL60 cells are an established model for study of myeloid cell development. We have used this model to demonstrate that transient and regulated expression of mutant ELANE in HL60 cells interrupts myeloid differentiation and causes cell death via accelerated apoptosis in cells of the myeloid lineage. We have also demonstrated that an inhibitor of enzymatic activity of neutrophil elastase (NE), developed by Merck-DuPont pharmaceuticals in the 1980s, partially corrected the defect induced by mutant ELANE expression in these cells . In collaboration with Nasri et al., we recently reported that knockdown of ELANE in HL60 cells expressing the mutant gene can restore myeloid cell development and functions . These results suggest that both NE specific inhibitors and
genetic manipulation (ELANE KO or mutation correction) are promising novel treatments for ELANE associated neutropenia. Because of the importance of these results for patients and because of the diversity of mutations in ELANE that cause these diseases, we have expanded our studies in HL60 lines expressing different ELANE mutants and comparing the effects of the elastase inhibitor versus knockout (KO) of ELANE on myeloid cell differentiation and function.
We analyzed the therapeutic effects of MK-0339 NE inhibitor and genetic ELANE KO by examining two key characteristics of myeloid cells that underline in the basis of normal hematopoiesis: cell survival and cell differentiation. To evaluate the safety, i.e., retention of critical functions, with these two therapeutic approaches (NE inhibitor and genetic KO) we examined the antimicrobial capacity of these cells by measuring chemotaxis and bacterial killing.
Materials and Methods
HL60 cells (kindly provided by Dr. Steve Collins, Fred Hutchinson Cancer Research Center, Seattle, WA). Cell lines were maintained at 37°C 5% CO2 incubator in RPMI supplemented with 10% fetal bovine serum (Avantor Seradigm), 2 mM L-glutamine and Pen Strep (Thermo Fisher Scientific). Myeloid differentiation of HL60 cells was stimulated by adding 2 uM all-trans retinoic acid (ATRA) and culturing for 6 days. NE inhibition was achieved by addition of 1 uM MK- 0339 in the culture on the first day of differentiation .
Cytospins and staining
8.0x104 HL60 cells differentiated as above were spun onto Cytoslide microscope slides (ThermoFisher) using a Cytospin 4 low speed cytocentrifuge (Thermo Scientific), then stained with the Kwik-Diff staining system (Thermo Shandon) following manufacturer’s recommendations. Microphotographs were taken on a LEITZ LABORLUX S polarizing light microscope at 400X magnification using a Nikon DSLR digital camera.
Generation of ELANE mutant P139L, C151Y, G214R and
ELANE KO HL60 cell lines
We used CRISPR/Cas9 gene editing technology with homology directed repair (HDR) to generate mutant ELANE knock-in (KI) P139L, C151Y and G214R HL60 stable cell clones. We used Zhang lab’s CRISPR Designtool (http://crispr.mit.edu/) to identify a guide RNA to target near the desired mutation with minimal off-target effects. This guide was cloned into the pSpCas9(BB)-2A-GFP vector (Addgene 48138).
The Guide-it sgRNA Screening Kit (Clontech) was used to test the efficacy of different single guide RNAs (sgRNAs) in vitro prior to using them in HL60 cells. A 100nt single-stranded DNA oligonucleotide (ssODN) was designed as an HDR repair template, containing the desired mutation with ~50 nt of homology on either side of the predicted cut site. HL60’s were co-transfected with the ssODN repair template and the Cas9 vector containing the sgRNA using electroporation (Neon transfection system, ThermoFisher). Cas9 expression was confirmed by fluorescence microscopy using anti-Cas9 polyclonal and biotinylated Goat anti-Rabbit secondary antibodies (Epigentek) with AlexaFluor 488 streptavidin detection. 24 hours after transfection, the cells were single-cell sorted using GFP selection into two 96 well plates containing 50% conditioned media. The cells were allowed to expand for three weeks. After the first week, the media was refreshed every 2-3 days. After three weeks of growth, a direct PCR kit (Clontech) was used to screen the colonies and confirm mutations.
ELANE total KO was performed by using a custom designed human gene knock-out kit for ELANE (GKO_HS3_ELANE) produced by Synthego. Clonal selection and identification were performed as described above for ELANE KI clones.
Flow cytometry analysis
The survival rate of cells during myeloid differentiation was evaluated by labeling with APC conjugated annexin V and PI (Biolegend) and analyzed by FACS. Cell debris and necrotic cells were gated out, and the percentage of annexin V-positive cells was expressed as the number of apoptotic cells/total number of cells.
Myeloid differentiation of HL60 cells was analyzed by flow cytometry using CD11b-APC (130-110-554) and CD15-VioBlue (130-113-488) cell surface antibodies (Miltenyi Biotec). Cell debris was gated out by using a zombie yellow viability kit (Biolegend, 423103)
Western blot analysis
NE protein loss in ELANE KO clones was confirmed by western blot analysis using anti-NE monoclonal antibody (Abcam, #121260, secondary: Abcam #205718) with actin specific monoclonal antibody as a loading control (Santa Cruz, #47778, secondary: VWR #NA931V). The unfolded protein response (UPR) master regulator protein GRP78/BiP was detected by western blot analysis using anti-BiP rabbit antibody (Cell Signaling, 3183S, secondary #7074).
6.5 mm Transwell plates with 5.0 μm pore inserts (Corning, #3421) were used to evaluate chemotaxis. IL-8 at 10 ng/ml was added to the lower chamber as the chemoattractant. 3 x104 HL60 cells differentiated for 6 days were placed in the upper chamber. Cells that had migrated through the membrane were counted in the lower chamber after 20 min of incubation.
Bacterial killing assay
A real-time luminescence based bacterial killing assay was developed to compare killing ability by differentiated granulocytes. HL60 cells were differentiated for 6 days as described above. The resultant granulocytes were incubated in a white 96 well flat-bottom tissue culture plate (Costar, #3917) at 1x105 or 5x104 cells per well in the presence of 2x105 luxCDABE-expressing bacterial cells. E. coli strain DH5alpha
transformed with a modified bacterial luciferase gene (luxCDABE) from Photorhabdus luminescens30 (addgene,
#14080) was opsonized in HBSS CaCl2+ MgCl2+, 20% FBS
for 30 minutes at 37oC with slow rotation in a hybridization
oven (Fisher Scientific, #13-247-20). The opsonized bacteria
were diluted to an OD of 0.04 in HBSS CaCl2+, MgCl2+ 10% FBS and added to differentiated HL60’s at a 2:1 ratio. The plate was returned to the hybridization oven and incubated for 2.5 hours. Light emission in relative light units (RLUs), was measured using a luminometer (CentroXS3 LB960). Wells without differentiated HL60s (E. coli only) and with 10 ng/
ml of the phagocytosis inhibitor Cytochalasin D (Santa Cruz
Biotec, #sc-201442) served as controls. Changes in RLU were
calculated and graphed.
We first generated mutant ELANE KI P139L, C151Y and G214R HL60 stable cell clones. The heterozygous amino acid substitutions in P139L and C151Y are consistent with naturally occurring mutations in patients with ELANE associated neutropenia. The mutant G214R cell line harbors a homozygous substitution, which was intentionally used in this study to see the cumulative effect of the mutant protein produced by both alleles in the complete absence of the normal NE (Figure 1A). Interestingly, the expression of the NE in these mutant cell lines is very similar to the w-type. Using a commercially available customizable gene knock-out (KO) kit (Synthego), we also successfully developed an HL60 cell line completely lacking w-type NE for these comparative studies (Figure 1B).
The resultant cell lines, in the undifferentiated state, demonstrate very similar cell growth and cell viability in culture compared to the w-type. ATRA induced myeloid differentiation of these mutant cell lines during 6 days in culture results in a typical block of differentiation in the promyelocytic stage and accelerated apoptosis which correlates with what is observed in patients with ELANE associated neutropenia (Figure 1C).
CRISPR/Cas9 edited ELANE KI and KO lines, as well as w-type HL60 cells, were cultured with ATRA as described above to induce myeloid differentiation. At day 5, cells were labeled with Annexin V and cell apoptosis was measured by flow cytometry. As expected, accelerated apoptosis was observed in all three ELANE mutant cell lines (P<0.0001) compared to the w-type. Addition of 1 uM MK-0339 significantly reduced the impaired cell survival. (P<0.0001). Survival of the ELANE KO line was very similar to w-type (Figure 2A). The unfolded protein response (UPR) in these cell lines was assessed by western blotting using a polyclonal antibody to GRP78/BiP. GRP78/BiP protein expression was noticeably upregulated in all three mutant cell lines compared to w-type. Addition of 1uM MK-0339 normalized this abnormality in these cell lines. GRP78/BiP protein expression in the KO cell line was not altered compared to w-type (Figure 2B).
CRISPR/Cas9 edited ELANE KI and KO lines, as well as the w-type CTRL HL 60 cells were treated with 2 uM ATRA to induce myeloid differentiation and cultured as described above. At day 5, cells were labeled with CD11b and CD15 granulocytic differentiation surface markers and examined by flow cytometry. The proportion of CD11b+/CD15+ cells was significantly lower in all three mutant cell lines compared to the w-type (P<0.0001), showing impairment of myeloid differentiation consistent with that observed in patients with ELANE associated neutropenia. Addition of 1 uM MK-0339 restored the impaired cell differentiation. There were no significant differences in the proportions of cells expressing these surface markers between the w-type and ELANE KO cell lines (Figure 3).
CRISPR/Cas9 edited ELANE KI lines cultured with or without MK-0339, the KO line, and w-type CTRL HL60 cells were cultured for 6 days in the presence of 2uM ATRA to induce myeloid differentiation. The migration of the resultant cells toward 10 ng/ml IL-8 was examined using a transwell system. Chemotaxis was significantly diminished in all three mutant lines (p<0.0002), and was partially restored by treatment with MK-0339. There were no statistically significant alterations in chemotactic capacities of ELANE genetic KO or MK-0339 inhibitor treated HL60 cells compared to untreated w-type (P>0.0831) (Figure 4).
Bacterial killing assay
CRISPR/Cas9 edited ELANE KI lines cultured with or without MK-0339, the KO line, and w-type CTRL HL60 cells were cultured for 6 days in the presence of 2uM ATRA to induce myeloid differentiation. The bacterial killing capacity of these cells was examined using the luxCDABE gene cluster modified E. coli strain as a luciferase reporter. We observed no significant differences in the bactericidal capacities of inhibitor treated and KO cell lines compared to the w-type (P>0.9996) (Figure 5). Inhibitor-free mutant lines exhibited higher killing than inhibitor treated lines, KO and w-type, but this difference was only statistically significant for the G214R line compared to w-type (p=0.03) (See Discussion).
Human promyelocytic HL60 cells are an established model for studying myeloid cell development and diseases. HL60 cells grow well in basic tissue culture medium with a cell doubling time of two days. They demonstrate high efficiency and recovery rates during transfection (electroporation). In the presence of DMSO or all trans retinoic acid (ATRA) HL60 cells easily differentiate towards functional granulocytes in 4-6 days, with antimicrobial characteristics and morphology comparable to human neutrophils [14-20].
Bone marrow cells from patients with cyclic and severe congenital neutropenia, particularly from patients with mutations associated with leukemia or poor response to G-CSF, are not readily available. The diseases are rare, bone marrow aspiration is painful and expensive, and patients with mutations causing more severe disease, e.g., G214R and C151Y, are usually transplanted or die from infections or leukemia. Mouse models of ELANE associated neutropenia have failed to recapitulate the human phenotype or serve as a model for the disease. There is an increasing interest in using the zebrafish platform as a model for this disorder [21,22]. We have previously successfully used patient derived iPS cells for studying neutropenia . Although these cells are directly derived from patients, there is always a concern about the artificial nature of the methods used to produce them. Tissue culture techniques with these cell lines are very complex and results can vary significantly. Lineage differentiation assays using iPSCs are lengthy and do not always consistently result in homogeneous cell production [23-27]. Regardless of their availability and ease of use, HL60 cells have limitations when studying human hematopoiesis. HL60 is a leukemic cell line with acquired proliferative characteristics due to mutations in some key tumor suppressor genes. Undifferentiated HL60 cells lack secondary granules and are missing important granule and surface marker proteins specific to mature neutrophils. With differentiation in DMSO or ATRA, these cells acquire antimicrobial capacity, but these functional properties are significantly less than for normal blood neutrophils [18-20,28]. In this study we have observed another potential limitation of working with HL60 cells. When examining the bacterial killing capacity of ELANE mutant KI lines differentiated using ATRA we detected higher bacterial killing in cultures without added inhibitor (Figure 5). We hypothesize that this is because culture with ATRA alone induces partial differentiation followed by cell death in the mutant-expressing lines. The dying cells release enzymes and cellular DNA in a “NET effect” may contribute to the bacterial killing. It is also possible that apoptotic cells have more adhesive cell membranes, causing the bacteria to stick to the cell surface which reduces the luminescence signal.
CRISPR/Cas9 gene editing techniques have made precise manipulation of the genome feasible. The current study required creation of stable cell lines harboring mutant ELANEs or ELANE KO clones. This would not have been possible using primary bone marrow cells. Using single cell sorting, we have developed stable HL60 cell lines expressing P139L, C151Y and G214R single point ELANE mutations. Although it has been reported that the total amount of NE protein is lower in cells of neutropenic patients , our ELANE KI mutant cell lines express an equal amount of NE compared to w-type. This enables these lines to express sufficient mutant protein to result in measurable phenotypic changes. These specific amino acid substitutions were chosen based on clinical studies of ELANE associated neutropenia. The P139L mutation usually manifests a mild phenotype compared to C151Y and G214R, which are known to cause severe disease with frequent complications and a high probability of transformation to MDS/ AML.  It is important to note that the G214R expressing cell line harbored a homozygous mutation. Naturally occurring homozygous ELANE mutations have not been observed in the human population. Interestingly, we did not observe a more severe phenotype in this cell line compared to the other mutant lines. This could be due to the confining conditions of in vitro experiments vs the natural bone marrow environment in patents, or the limiting conditions of the HL60 cellular platform.
It is important to note that when cultured in the immature state, the CRISPR/Cas9 edited mutant ELANE HL60 clones used in this study demonstrate very similar characteristics in terms of cell growth and cell viability compared to w-type. ATRA induced myeloid differentiation over 6 days promotes transition to a specific neutropenic phenotype in these mutant cell lines which correlates with what is observed in patients with ELANE associated neutropenia.
We have previously reported that the ELANE KO strategy improves survival of HL60 cells expressing mutant NE. We have also observed that NE inhibitors have similar effects . Nasri et al., recently reported that CRISPR/Cas9 mediated ELANE KO improves myeloid differentiation and maturation in neutropenia patient derived bone marrow primary cells .
Another approach for genetic correction of ELANE associated neutropenia is the CRISPR/Cas9 mediated direct correction of ELANE mutations utilizing the cell’s homology directed repair (HDR) machinery. At first glance, this methodology is very promising as it repairs the nucleotide mutation and does not result in ELANE KO. However, there are some serious limitations to this approach. First, the efficiency of HDR after CRISPR/Cas9 mediated double-strand break is very low and might not be feasible for clinical applications. Secondly, ELANE heterozygous gain-of-function mutations are distributed throughout all five exons of the gene, and it would require specific guide and repair templates for each patient-specific genotype. There is also a serious concern with widely used adeno-associated viral (AAV) delivery methods of the donor repair template and its use for clinical applications. Moreover, a gene editing approach focused on the correction of ELANE mutations may result in novel mutations due to low efficiency and inadequate homology dependent repair of double strand cuts and thus to novel variants of NE protein.
In this study we compared ELANE KO cells expressing no NE to cells expressing mutant NE in the presence of the cell permeable NE inhibitor, MK-0339. In general, the phenotype rescue effects were similar. The inhibitor improved cell survival and proliferation for cells expressing any of the 3 mutations. The inhibitor was also permissive of maturation for all 3 cell lines. The effect was very similar to the KO cell line. There was no significant difference in survival or myeloid differentiation between the ELANE KO line compared to w-type. These results suggest that either knocking out both alleles of the ELANE gene or inhibiting NE with a cell permeable inhibitor might have similar clinical benefits. We acknowledge that we have examined only 3 of the more than 100 different ELANE mutations associated with cyclic and congenital neutropenia. We believe they represent the diversity of mutations, but will continue to investigate other mutations and the basic mechanisms underlying the biologic effects of neutrophil elastase inhibitors.
The two knock-down approaches used in these experiments result in either total removal of NE (genetic KO) or greatly diminished enzymatic function (chemical inhibitor). Therefore, we assessed whether granulocyte effector functions were impaired in differentiated KO or inhibitor treated mutant NE lines. Chemotaxis and bactericidal assay results with KO and inhibitor treated lines were similar to w-type. It may be surprising that KO and inhibition of neutrophil elastase have no effect on bactericidal activity. It is obvious that the current study and previous studies have examined only a few organisms and biological processes which involve neutrophils and their proteases. It is also unclear whether NE or other of the related proteases have effects on remodeling of myeloid precursors as they progress from early progenitors to mature neutrophils.
With the total KO strategy, there is a valid concern for the complete elimination of normal NE. ELANE associated neutropenia is an autosomal dominant disease and there is a functional normal NE product translated from the healthy allele. Total ELANE KO completely eliminates the expression of both healthy and mutant variants of NE protein. There are a number of contradictory studies in the literature about the role of NE. Some investigators have demonstrated a non-redundant role of NE protein, showing it has an essential role in antimicrobial defense during innate immunity by degrading outer bacterial membrane proteins and modulating inflammatory cytokines [30-33]. NE knock-out mice displayed increased susceptibility to sepsis due to infection with gram negative bacteria . NE also has a role in antifungal defense . There is data that NE takes an important role during the formation of neutrophil extracellular traps (NETs). It has been demonstrated that ELANE KO, and more interestingly, NE inhibitor treated granulocytes, exhibit abnormal NET formation . Other investigators state
that the antimicrobial role of NE is highly redundant and there
are a number of other serine proteases expressed by white
blood cells that have similar functions during innate immune
response [37-39]. The highlight is Papillon-Lefevre Syndrome
(PLS) which is the only known human disorder causing NE
deficiency. This is a rare autosomal recessive genetic disease
caused by mutations in the DPPI gene encoding cysteine
protease cathepsin C/dipeptidyl peptidase I (DPPI). This
enzyme mediates N-terminal processing of NE as well as other
serine proteases: cathepsin G and Proteinase 3. PLS patients
display a severe reduction in the activity and stability of all
three serine proteases. Interestingly, patients with PLS have
no bacteriocidic deficiencies suggesting the redundant role of
these serine proteases for killing common bacteria .
The other serious concern for the ELANE KO strategy is off target effects. Even with recent improvements it has been demonstrated that CRISPR/Cas9 genome editing can hit off target sites, generate DNA double strand breaks and introduce unintended mutations . The use of computational bioinformatics along with next generation sequencing (deep sequencing) is essential for reducing off target effects and selecting suitable editing components in order to minimize the occurrence of off target effects.
Considering the disputed role of NE in host immune defense mechanisms, as well as the comparable results of NE inhibition vs ELANE KO observed in this study, we believe that pharmacological inhibition of NE is more favorable and more readily feasible for clinical applications than a genetic KO approach. By its nature, chemical inhibition is to some degree incomplete and in many cases is reversible. With chemical inhibition, the manipulation is on the protein level but not on the genetic level. The gene remains functional and there should always be some level of normal NE product expressed, which would be well controllable by administered dose correction of the NE inhibitor.
There is a long history of studying NE inhibitors as antiinflammatory drugs. We first reported the potential use of these inhibitors to treat ELANE associated neutropenia more than a decade ago. Steadily we and others have accumulated increasing evidence that cell permeable inhibitors may be an effective treatment, but thus far there have been no clinical trials to demonstrate clinical effects or benefits. The dosing strategies to achieve clinically beneficial inhibitory concentrations in the bone marrow are not known. The in vitro concentrations of the inhibitor used in this study (MK0339), however, are comparable to the blood concentrations achieved in previous clinical trials in normal subjects and patients with various inflammatory diseases [42-44]. Thus, we believe there is sufficient evidence from our in vitro work and previous clinical trials to justify small scale, short-term phase 2 investigations of this inhibitor and possibly other NE inhibitors.
It is still very early in the development of alternatives to G-CSF as treatments for ELANE associated neutropenia. Work advancing the knockout strategy is advancing rapidly. These studies involve either knocking out both alleles to produce KO cells similar to those used in this investigation or single allele knock out to remove only the mutant allele [45,46]. Clinical applications will require leukapheresis for collection of patients’ hematopoietic stem cells, ex vivo editing and autologous transplantation with sufficient numbers of progenitor cells to produce sufficient neutrophils to correct neutropenia. There is precedent for being successful with each of these steps toward permanent correction of neutropenia. Successful application will also depend upon avoidance of significant off target effects of the gene editing procedures. In the near term, it would be best to conduct at least a preliminary trial of an orally absorbed, cell permeable inhibitor of neutrophil elastase.
We developed an HL60 cell model to study the mechanisms of and potential treatments for ELANE neutropenia. We studied three stable HL60 clones expressing ELANE mutants, i.e., P139L, C151Y and G214R, mutations associated with different clinical outcomes. All three mutant clones produced few mature neutrophils on exposure to ATRA, with similar apoptosis profiles. These abnormalities were abrogated by adding the cell permeable elastase inhibitor MK-0339 to the cultures. Both treatment of the mutant clones with this inhibitor and ELANE KO led to formation of neutrophils with comparable chemotactic and bactericidal capacities. These studies suggest that both strategies have great potential for the treatment of cyclic and congenital neutropenia. We conclude that an orally absorbed, cell permeable inhibitor of neutrophil elastase, if proven safe and effective in a clinical trial, is a good alternative to G-CSF or gene editing to treat ELANE neutropenia.
Conflict of Interest
V.M., M.L.K., B.F., I.A. and T.P. declare no conflicts of interest. D.C.D. is an inventor on the joint University of Washington– Merck patent for the use of inhibitors of NE to treat inherited severe neutropenia. D.C.D. is also a consultant and receives research support from Amgen, a manufacturer of G-CSF, mentioned in the article.
This research was funded by NIH/NHLBI grant number
1R01HL151629-01, David C. Dale, PI
We gratefully acknowledge support from Merck in providing
the inhibitor drug used for this work and Peter Sabo for the assistance with the bacterial killing assay.
Author Contribution Statement
V.M., D.C.D. and M.K. organized and designed this research study and wrote the report. V.M., M.K., B.F., I.A., T.P. conducted laboratory studies and data analysis.
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