Commentary Open Access
Volume 3 | Issue 3 | DOI: https://doi.org/10.33696/cancerimmunol.3.051

Anti-PD1 Therapy in Lynch Syndrome-associated Recurrent Glioblastoma

  • 1Senior Associate Consultant, Assistant Professor, Departments of Neurology and Neurosurgery, Mayo Clinic Florida, 4500 San Pablo Rd. S., Jacksonville, FL 32224, USA
+ Affiliations - Affiliations

*Corresponding Author

Wendy J Sherman, Sherman.Wendy@mayo.edu

Received Date: July 07, 2021

Accepted Date: August 23, 2021


When a patient is diagnosed with a glioblastoma, they are often blindsided with the diagnosis, experiencing symptoms typically developing over only days to weeks and then often undergoing a craniotomy within days of presentation, only to then be given a terminal diagnosis. Patients often ask “why me,” “what did I do to cause this to occur,” and “is this hereditary?”

Less than 5% of glioblastomas result from a hereditary syndrome. While not common, they do occur and perhaps may be under-recognized if family history is not known. Now, with more frequent germline testing done as a component of next generation tumor sequencing, it is hypothesized that these hereditary syndromes are better detected. This improved detection is not only beneficial for screening family members and screening the patient for other associated malignancies, but this opens up an opportunity for us as clinicians and scientists to better understand the tumorigenesis of glioblastoma in hereditary syndromes, which in turn may offer individualized treatment regimens.

The use of immunotherapy in glioblastoma has continued to present challenges despite its success in several malignancies. Immune checkpoint inhibitors have changed the treatment landscape for many cancers, including melanoma, renal cell carcinoma, head and neck squamous cell carcinoma, and others [1], but unfortunately have not shown the same results in glioblastoma. Checkmate 143 was a phase III clinical trial investigating nivolumab compared to bevacizumab at first recurrence in patients with glioblastoma. This study did not meet the primary end point of improved overall survival with median overall survival comparable in both arms [2]. This lack of success has been attributed to several factors specific to glioblastoma. While we now know the central nervous system is subject to active immunosurveillance and immune responses [3], glioblastoma has been proven to induce both local and systemic immunosuppression thereby evading the immune response. This appears to result from factors intrinsic to glioblastoma as well as factors related to the host response itself [4,5]. There are numerous ongoing efforts to overcome or circumvent the glioblastoma-induced immunosuppressive state through combinatory approaches of immunotherapy.

We recently reported the case report of a patient with Lynch Syndrome and glioblastoma treated with nivolumab, an immune checkpoint inhibitor [6]. Lynch syndrome, also known as hereditary nonpolyposis colorectal cancer, is an autosomal dominant disorder caused by mutations in mismatch repair (MMR) genes. Patients with Lynch syndrome are at high risk of synchronous cancers, with the risk level dependent on which MMR mutation is harbored. Glioblastomas have a known association and occurrence with Lynch Syndrome, with an estimated risk of 1-6% of brain malignancy in patients with Lynch Syndrome [7]. However, the literature is sparse regarding incidence and null regarding unique treatments related to glioblastoma in the setting of Lynch Syndrome.

Immune checkpoint inhibition has proven great success initially in colon cancer with microsatellite instability (MSI) or mismatch repair deficiency (MMR-d), as in Lynch Syndrome [8], and later with other malignancies associated with the same features [9,10]. Its use however in glioblastoma as a whole, not selecting out for MSI or MMR-d, has been disappointing to say the least.

Our patient had a known diagnosis of Lynch Syndrome, diagnosed after a colon cancer diagnosis 10 years prior to her glioblastoma presentation. She was initially diagnosed with isocitrate dehydrogenase (IDH) wild type glioblastoma with O6-methylguanine- DNA methyltransferase (MGMT) unmethylation after craniotomy for tumor resection, subsequently receiving radiation with concurrent temozolomide, but was unable to receive adjuvant temozolomide due to cytopenia. She was treated with tumor treating fields for 18 months. She then had recurrence at a distal intracranial location for which she again underwent craniotomy for tumor resection, with pathology again consistent with IDH wild type, high grade glioma, MGMT unmethylated. Her tumor was found to be microsatellite unstable with a high tumor mutational burden. She was subsequently re-irradiated and treated with off label nivolumab which was continued after radiation completion without recurrent after now 40 months of nivolumab treatment. Additionally, she is without other systemic malignancy, including without recurrence of her more remote diagnosis of colon adenocarcinoma.

Anghileri et al. simultaneously published a similar case of a Lynch syndrome-associated recurrent glioblastoma responding to nivolumab. This patient did not have known Lynch syndrome at the time of glioblastoma diagnosis, but was discovered through germline sequencing revealing a pathogenic mutation in the MSH2 gene. Their patient was reported as still receiving nivolumab 68 months after recurrence [11]. They performed mutation signature analysis of both the initial and recurrent tumor, reporting that both specimens demonstrated mismatch repair deficiency. Additionally, they reported that the mutational burden in their case was “significantly higher than that in most hypermutant GBM patient data in TCGA”[11]. Both our case report and that of Anghileri et al. correlate response to nivolumab with tumor mutational burden.

This is being further investigated in the currently enrolling Alliance trial evaluating nivolumab and ipilimumab in patients with recurrent glioblastoma demonstrating elevated tumor mutational burden [NCT04145115].

Both case reports of IDH wild type, glioblastoma in the setting of Lynch Syndrome responding to nivolumab highlight the importance of an individualized approach in managing patients with glioblastoma. It appears that the microsatellite instability harbored in our patient’s glioblastoma correlates with the response to immune checkpoint inhibition with nivolumab. These cases shed light not only on the potential use of immune checkpoint inhibition in glioblastoma with tumor features, or genetic syndromes, associated with response to the same therapy for other malignancies, but more broadly shed light on the need to individualize glioblastoma treatment, particularly in the setting of glioblastoma as a result of an inherited syndrome.

Unique in our case report was the use of checkpoint inhibition concurrently with radiation therapy. The combination of immunotherapy with radiation therapy has been shown to potentially boost the abscopal effect seen in other malignancies treated with radiation therapy. The abscopal effect was first described in 1953 by Mole, where he described an immune-mediated response to radiation by distant tumor cells resulting from immune system activation by antigen release during radiotherapy (Figure 1) [12].

More recently, there is a growing body of evidence supporting the role of immunotherapy in boosting the abscopal effect [13]. This is particularly of interest in the field of glioblastoma given the infiltrative nature of the disease and the high rate of recurrence just outside the radiation field. Further research is needed to investigate the dynamics between immunotherapy and radiation therapy in glioblastoma treatment.

There have been improved collaborative efforts in glioblastoma, a designated rare disease. However, glioblastoma in the setting of Lynch Syndrome represents an even rarer diagnosis necessitating international collaboration and perhaps a consortium effort to further investigate and treat glioblastoma in this setting. In writing our case report, we are putting forward an ask to researchers and clinicians to join in the effort to individualize therapy. With the changes first in 2016 to the World Health Organization Central Nervous System Tumor Classification and the more recent 2021 WHO update, the door to understand the variability of glioblastoma has been opened which will hopefully lead to tailored therapies such as we report in our Lynch Syndrome patient without progression on immunotherapy for her recurrent IDH wild type glioblastoma.


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