Research Article Open Access
Volume 2 | Issue 6 | DOI: https://doi.org/10.33696/immunology.2.059

A Native 51 kDa Leishmania Membrane Protein Revealed as a Novel Antigenic Candidate for Immuno-Diagnosis of Human VL and PKDL diseases

  • 1Infectious Diseases and Immunology Division, Indian Institute of Chemical Biology, Jadavpur, Kolkata-700032, India
  • #Authors contributed equally
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Corresponding Author

Nahid Ali, nali@iicb.res.in

Received Date: July 07, 2020

Accepted Date: September 15, 2020


Background: Visceral leishmaniasis (VL), a potentially devastating neglected tropical disease is a major health concern. Despite recent advances made in diagnosis of VL, an accurate and a reliable diagnostic biomarker is still needed.

Objective: This study primarily aims to characterize the 51 kDa protein of Leishmania donovani promastigote membrane antigens (LAg) for diagnosis of VL and post kala-azar dermal leishmaniasis (PKDL).

Methods: One of the antigenic components of LAg of molecular weight 51 kDa which was previously identified as elongation factor1-α (EF1-α), was electroeluted and confirmed with anti-EF1-α antibody through immunoblot assay. Sero-diagnostic potential of 51 kDa antigen and one of its B cell epitopes was accessed through ELISA. The 51 kDa was further elucidated for urine-based diagnosis of PKDL.

Results: In the present study 51 kDa antigen was investigated for reactivity with VL patients’ sera. A strong immuno-reactivity of this antigen was observed in ELISA demonstrating 98.27% sensitivity with VL sera and 93.33% specificity with endemic and nonendemic healthy controls as well as other diseases. Further a B cell epitope of 51 kDa antigen was found to be 100% sensitive and 93.33% specific in ELISA. Additionally, in another ELISA 51 kDa antigen showed 100% sensitivity and specificity with PKDL patients’ urine.

Conclusion: The data obtained from this study leverage the potential of 51 kDa antigen in diagnosis of VL and PKDL which can be developed as a rapid diagnostic test.


Diagnosis, Visceral leishmaniasis, Post kala-azar dermal leishmaniasis, Antigen, Immunological assays, ELISA, Electroelution

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