Journal of Cellular Immunology
ISSN: 2689-2812

Commentary - Journal of Cellular Immunology (2019) Volume 1, Issue 2

Proteomic Functional Signatures during the Priming of Human Th17 Cells

Mohd Moin Khan1,2, Robert Moulder1, and Riitta Lahesmaa1*

1Turku Bioscience Centre, University of Turku and Åbo Akademi University, FI-20520 Turku, Finland

2Turku Doctoral Programme of Molecular Medicine (TuDMM), Medical Faculty, University of Turku, Turku, Finland

*Corresponding Author:
Riitta Lahesmaa
E-mail: rilahes@utu.fi

Received date: September 09, 2019; Accepted date: September 24, 2019

Citation: Khan MM, Moulder R, Lahesmaa R. Proteomic Functional Signatures during the Priming of Human Th17 cells. J Cell Immunol. 2019; 1(2):25-28.

Copyright: © 2019 Khan MM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Quantitative Proteomics Characterization Deciphers New Rules of Human Th17 cells Differentiation Regulation

A combination of regulated responses toward pathogens and minimized autoimmune reactions is needed for the balanced function of the immune system. Amongst the immunologically important CD4+ lymphocytes, T helper 17 (Th17) cells help maintain homeostasis and provide protection against pathogens of fungal or bacterial origins [1,2]. Th17 cells are characterized by their ability to secrete interleukin (IL)-17, a pro-in lammatory cytokine, which has been associated with the pathogenesis of several in lammatory diseases. The latter include psoriasis, rheumatoid arthritis, in lammatory bowel disease and multiple sclerosis. Accordingly, the targeting of Th17 cells for the treatment of the human autoimmune diseases has received much interest [3-9]. Therefore, an understanding of the molecular mechanism of Th17 cell differentiation is important when developing new therapeutics against human in lammatory diseases.

To date, the characteristics of Th17 cells and changes related to Th17 differentiation have been described in transcriptome data sets from both human and mouse [10-16]. These studies revealed important candidates associated with the pathologies caused by Th17 cells, some of which were further studied [3,6,15,17-20]. Notably, our previous comparative careful analysis of RNA-sequencing data of 10 longitudinal samples taken during early Th17 differentiation in human and mouse showed very little overlap between the species [14]. To complement our previous study, we exploited quantitative proteomics to study dynamics of protein expression during early stages of human Th17 cell differentiation. Further, we looked into concurrence of Th17 proteomics with Th17 transcriptome as well as compared Th17 proteomics results between human and mouse.

In the January 2019 issue of iScience, we presented results from a combined analysis of the Th17 cell proteome and transcriptome in human [21]. Importantly, this novel proteomic study of human Th17 cells revealed notable differences and correlations previously not reported. In keeping with an earlier report, there were more striking differences in Th17 regulation between the mouse and human proteins [21]. For example, Tripathi et al. [21] demonstrated that the role of SATB1 in human Th17 cell differentiation was opposite to that observed in the mouse [22-24]. In human Th17 cells, SATB1 was found to negatively regulate differentiation, further emphasising the importance of human studies for translational research. Also, regarding the changes in the human Th17 proteome and transcriptome, comparison of the cells after 72 hours of polarization revealed a high level of overlap; for 93% of the proteins differentially abundant in Th17 cells relative to Th0 cells the detected mRNA transcripts were changed in a similar fashion.

Tripathi et al. [21] used a label-free liquid chromatography-tandem mass spectrometry (LCMS/ MS) approach to profile Thp, Th0, and Th17 cell subsets. Statistical analysis was made to identify proteins differentially expressed (DE) amongst these subsets. Consistent with our previous transcriptomics results [13,14] activation induced the greatest effect on abundance change, with the detection of 2050 and 3470 differential abundant proteins at 24h and 72h, respectively. The comparison between Th0 and Th17 cells identified 148 and 175 differentially regulated proteins at 24h and 72h, respectively. Several proteins previously reported to be differentially regulated during Th17 differentiation were confirmed. Importantly, changes were detected in a number of proteins with no previously known functions in Th17 cells. The latter included the upregulation of UHRF1, GK, ICAM1, KDSR, ATF3, IL4IT, APOD, VIM, PALLD, PTGFRN and KDM6A, and downregulation of ELF1, IL16, BTLA, LAIR1, UHRF1, ANXA1, NDFIP1 and NDFIP2. Future studies could aim at addressing the role of this unique set of proteins in Th17 cell differentiation and function.

Validation measurements, using the combined versatility of immunoblotting, flow cytometry and selected reaction monitoring mass spectrometry (SRM-MS), were made in additional samples to confirm changes in abundance. For example, ACSL4, FHOD1, radixin (RDX), smoothelin (SMTN) and PALLD expression were validated by SRMMS, and OASL and ATF3 by flow cytometry. Western blot analysis confirmed upregulation of BASP1, LMNA, ATP1B1 JUNB, SIRT1, CTNNA1, FAS, VIM, and IRF7, and downregulation of SATB1, SMAD3, STAT4, and ETS1 in Th17 cells. Amongst these, of particular interest for future studies on Th17 cell regulation and Th17 mediated immunopathology is for example ACSL4, a lipid metabolic enzyme that catalyzes addition of a coenzyme-A group to free long-chain fatty acids (Figure 1). Such are also the cytoskeleton proteins SMTN, RDX, and FHOD1, which are already known to play a key role in T cell activation and homeostasis by regulating cell adhesion, migration and T cell interaction with antigen presenting cells [25,26]

The proteomic analysis was made using whole cell lysates, and accordingly analysis of the Gene Ontology (GO) of the DE proteins indicated enrichment of a range of ontologies. The most enriched biological processes reflected cell stimulation and differentiation, i.e. response to external stimulus and cell surface receptor signaling pathway, as well as regulation of the immune system process and immune system development. Lipid metabolic processes were also highly enriched and represented with the previously reported differential regulation of VDR, MSMO1 and CYP51A1 [27,28], as well as other proteins previously not reported in relation to Th17 cells.

Detailed comparison was made between the proteomics of human and mice Th17 cells further emphasizing the dissimilarity of the two. Amongst the top 25 proteins DE in human and mouse only IL2RG was detected in both species. The protein IL2RG is a common receptor gamma chain, and a component of several interleukin receptors, including those of interleukin -2, -4, -7 and -21. Moreover, of altogether 758 differentially regulated proteins in human and 397 in mouse, only 33 were differentially regulated in the same direction, whereas 18 were regulated in an opposite fashion between the two species.

Overall, the study highlights the importance of comprehensive analysis of human cells to provide basis for translational research. Identification of targets that are regulated in a similar fashion in human and mouse points out the candidate human targets worth studying by using in vivo mouse models.

Acknowledgements

We would like to thank Academy of Finland (grants 292335, 294337, 319280, 292482 and 31444) and the Sigrid Jusélius Foundation for their support.

References