Commentary - Journal of Cellular Signaling (2020) Volume 1, Issue 2
DNA Nanotechnology Engineered Vesicle for Mimicking Biomolecular Signaling
Ruizi Peng1#*, Yingzi Ma2#, Yan Zhou1,Yongbo Peng1, Xiaofang Zheng1, Qiang Zhang1
1Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Bio-Sensing and Chemometrics,
College of Chemistry and Chemical Engineering, College of Biology, and Aptamer Engineering Center of Hunan Province, Hunan University, Changsha, Hunan 410082, China
2College of Life Science and Technology, Central South University of Forestry and Technology, Changsha, Hunan 410004,
#Equally contributed authors
- *Corresponding Author:
- Ruizi Peng
Received date: May 20, 2020; Accepted date: June 03, 2020
Citation: Peng R, Ma Y, Zhou Y, Peng Y, Zheng X, Zhang Q. DNA Nanotechnology Engineered Vesicle for Mimicking Biomolecular
Signaling. J Cell Signal 2020; 1(2): 35-37.
Copyright: © 2020 Peng R, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Bio-inspired strategy is kind of interesting to fabricate devices and perform dynamic operations . Various devices have been made, such as airplane, radar and submarine. In life science, as the fundamental entity, million years’ evolution enables cell becomes the most successful functionality. Thus, cell studies undoubtedly deepen our understanding of living systems, and it further bring some predesigned functionalities in living systems . Since Thomas Chang demonstrated the feasibility of artificial cells, scientists have constructed a number of artificial cell-like systems for mimicking cellular molecular signaling . It requires at least three key components towards engineering artificial cell: (i) a metabolic machinery that captures energy and resources for cell living; (ii) a membrane component that keeps the living entity stable and separated from the environment; (iii) a genetic system that processes and transfers information heritance [4,5].
DNA, a genetic molecule, shows great potential in nanotechnology and molecular engineering. Based on DNA hybridization and chain displacement reaction, dynamic DNA nanotechnology shows precisely molecular operations with nanomachines , such as DNA logic gate, DNA motor, DNA walker, DNA computing and DNA reaction networks . For structural DNA
nanotechnology, topological nanostructures have been designed and constructed by Watson-Crick base
pairing, such as DNA dendrimer, DNA framework, DNA
hydrogel, and DNA origami [8,9]. In a recent decade,
the advancements in DNA nanotechnology inspired us
to develop an artificial cell with a DNA engineering way
. Several years ago, a biomimetic giant vesicle was
generated from detached cancer cells including HeLa and
HepG2 cells, which preserved the host cellular properties
. Generally, researchers employed synthetic
phospholipid bilayer as the membrane models to study
cell membrane biology . Compared with the synthetic
phospholipid vesicle, our giant vesicle possesses the host
cell membrane properties and maintain cellular size.
Based on such cell-mimicking giant vesicle, we developed
the approach of engineering artificial cell through DNA
nanotechnology. We achieved reversible regulation of
nanoprisms via DNA hybridization and DNA strand
displacement reaction on cell-mimicking surface .
Then, a DNA cascade reaction was constructed on cellmimicking
surface for mimicking cellular adaptivity .
However, the homeostasis of life organism requires a
signaling system to transmit information and feedback
environmental stimuli. Surface molecular engineering
needs to rational interact with the encapsulated system.
In the nature, biomolecular signaling network consist of a series of spatiotemporal and ordered chemical reactions, regulating different biological molecules to maintain living organisms. A key challenge of engineering molecular signaling system is to design and construct a rational integrated functional module to regulate the cascade of molecular events. Therefore, it is attractive to construct a prototype cell with artificial reaction network as the computational core and be able to perform programmed functions . As a proof-of-concept, in
February, we reported the new strategies of engineering
artificial cell by DNA nanotechnology based on our cellmimicking
giant vesicle. A DNA-based artificial molecular
signaling system was constructed on our biomimetic giant
vesicle, which was derived from a mammalian cell .
As shown as in figure 1, a switchable nanochannel was
engineered on membrane for materials and information transferring. Given that adenosine triphosphate (ATP) is a
canonical energy molecule that triggers cellular machines
and drives metabolic reactionsin physiological and
pathological reactions, we designed an ATP-responsive
DNA nanogatekeeper spanning the membrane, and it
serves as the bridge transmitting information between
outside and inside of the vesicle. Then, we constructed an
encapsulated information processing system. The DNA
cascade network was rationally constructed for mimicking
cellular signaling pathways. The whole system consists
of on-membrane DNA nanostructure and encapsulated
DNA signaling network, mimicking the cellular signal
reception, transmission and response.
Cellular signaling networks play an important role in cell growth, communication, proliferation, and death. Rapid development of DNA nanotechnology has attracted attention of engineering molecular signaling. In biology, proteins and their interactions play a predominate role in signaling pathway. The strategy of DNA nanotechnology could provide another way to construct an artificial cell. In the future, through precise regulation of DNA nanotechnology, artificial signaling might build powerful applications.
Conflicts of Interest
Authors declare no conflict of interest.
The authors acknowledge the support from National Postdoctoral Program for Innovative Talents of China (BX20190111).
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