Perinatal brain injury is an important clinical and socioeconomic entity. It is a syndrome of impaired brain function in the early days of life, and it is a consequence of inadequate brain oxygenation before, during or shortly after birth, with high mortality rates and early and late morbidity rates.

As to better understand and characterize the dramatic differences in antioxidant response of human cells harboring mutations in the frataxin gene responsible for Friedreich's ataxia (FRDA), we studied primary cultures of skin fibroblasts derived from five FRDA patients with different major frataxin gene expansion sizes. Since oxidative stress has been previously established to play a critical role in FRDA, among the many enzymes that may modulate oxidative stress sensitivity, we selected some that have previously been shown to be critical in oxidative stress.

Few efforts were made to conduct a comprehensive analysis of potential genes within the action of exercise therapy on cerebral infarction (CI). We used RNA sequencing (RNA-seq) and weighted gene co-expression network analysis (WGCNA) to provide more important insights information than the conventional single-gene analyses. We performed RNA-Seq on the macroscopically preserved and lesioned SD rat CI model (N=4 pairs).

Hypoxia-inducible factor 1-alpha (HIF-1α) plays a critical role in regulating cellular responses to low oxygen levels. In our study, we transfected HIF-1α mRNA into human dermal fibroblasts and assessed its biological activity by measuring the upregulation of downstream angiogenic genes, aiming to investigate the delivery mechanism of HIF-1α mRNA and its functional impact. In this short communication, we will describe our transfection methodology, present the results we obtained, and discuss the potential implications of these findings for future therapeutic applications.