Commentary Open Access
Volume 4 | Issue 4 | DOI: https://doi.org/10.33696/immunology.4.144

Manufacturing of Human T-lymphoid Progenitors from Two Different Hematopoietic Stem Cell Sources and Perspective for New Immunotherapies

  • 1Laboratory of Human Lymphohematopoieisis, Imagine Institute, INSERM UMR 1163, Université Paris Cité, Paris, France
  • 2Smart Immune, Paris, France
  • 3Lab Tech Single-Cell@Imagine INSERM UMR 1163, Paris, France
  • 4Department of Diagnostic Sciences, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium
  • 5Cancer Research Institute Ghent, Ghent, Belgium
+ Affiliations - Affiliations

Corresponding Author

Pierre Gaudeaux, pierre.gaudeaux@institutimagine.org

Received Date: August 12, 2022

Accepted Date: August 24, 2022


The adaptive immune system depends on the efficient response of lymphocytes, protecting the organism from infection or malignant diseases. T-cell immunodeficiency, innate or acquired, put the patient at risk for developing opportunistic infections and cancers. We previously described a novel approach to overcome the major limitations of T-cell immunotherapy and hematopoietic stem cell transplant (HSCT) by transplanting human T-lymphoid progenitors (HTLP), with the aim to achieve shortened immune reconstitution and fully functional naïve T-cell repertoire in immunodeficient or immunocompromised patients. By complementing our DL4-based cell culture with TNFα we developed and scaledup clinical strategies involving hematopoietic stem and progenitor cells (HSPC) differentiated into T-lymphoid progenitors. We discuss here recent advances made in the characterization of different cell sources used as starting materials for T-lymphoid progenitors manufacturing, as well as gene modification of these cells, highlighting new perspectives for the development of therapeutical strategies.


Clinical immunology, Immune reconstitution, Immunodeficiency disorders, Immunotherapy, Stem cell, T-cell progenitors, Thymus, Transplantation immunology

Author Information X