Research Article Open Access
Volume 1 | Issue 1 | DOI: https://doi.org/10.33696/Nanotechnol.1.004

Bioconjugation of AuNPs with HPV 16/18 E6 Antibody through Physical Adsorption Technique

  • 1Department Molecular Biology and Biotechnology, Pan African University Institute of Science, Technology and Innovation Juja, Kenya
  • 2Department of Medical Laboratory Sciences, Jomo Kenyatta University of Agriculture and Technology, Juja, Kenya
  • 3Department of Medical Microbiology, Jomo Kenyatta University of Agriculture and Technology, Juja, Kenya
  • 4Department of Human Pathology, University of Nairobi, Nairobi, Kenya
+ Affiliations - Affiliations

Corresponding Author

Lucy Muthoni Mwai, luciemwai@gmail.com

Received Date: April 24, 2020

Accepted Date: May 09, 2020


Gold nanoparticle (AuNP) bioconjugates are increasingly being utilized in biomedicine due to their low toxicity on biological tissues and unique electronic and chemical properties. They have been utilized in several biological applications, namely the manufacture of nanomaterials, biosensing, electron microscopy, and drug delivery systems. Mainly, immuno-assays often employ gold nanoparticles (AuNPs) to enhance the detection of a biological component. This paper presents a study on the bioconjugation of AuNPs with Horse Radish Peroxidase conjugated Human Papilloma Virus 16/18 Early 6 antibodies (CIP5) against Early 6 (E6) oncoprotein that is overexpressed in cervical carcinoma progression through physical adsorption. This bioconjugate can be employed in a diagnostic immunoassay for cervical cancer screening. The study also demonstrated that the antibody pI, gold colloidal solution pH, and amount of antibody determine the generation of stable Antibody–AuNPs bioconjugates


Bioconjugates, Gold nanoparticles, Nanobiotechnology, Human papillomavirus virus, Early 6 (E6) protein, Immunoassay

Author Information X