Research Article Open Access
Volume 2 | Issue 3 | DOI: https://doi.org/10.33696/diabetes.1.023

Anti-diabetic Potential of Aqueous Extract of Moringa oleifera, Ocimum gratissimum and Vernonia amygdalina in Alloxan-Induced Diabetic Rats

  • 1Department of Microbiology, University of Lagos (UNILAG), Akoka, Lagos, Nigeria
  • 2National University of Ireland Galway, Ireland and University of Limerick, Ireland
  • 3Department of Cell biology and Genetics, University of Lagos (UNILAG), Akoka, Lagos, Nigeria
  • 4Department of Health and Health Care Administration, Swami Rama Himalayan University (SRHU), Dehradun, India
+ Affiliations - Affiliations

Corresponding Author

Ayobami AL, ayobamiadesiyan123@gmail.com

Received Date: August 15, 2020

Accepted Date: October 15, 2020


The incidence of diabetes mellitus (DM) is increasing globally, and it is a major source of concern. This study was undertaken to assess the antidiabetic effect of the aqueous extract of Moringa oleifera, Ocimum gratissimum and Vernonia amygdalina. Sixty adult Wistar rats with body weight of 120-150 g were randomly assigned to groups of five rats each (n=12). Group 1 served as normal control; Groups 2-5 were diabetic groups; group 2 served as negative control; group 3-5 received 100, 200 and 400 mg/kg of triherbal formula, respectively. The body weight (BW) and fasting blood glucose level (FBSL) of the rats were monitored weekly. At the end of the experiment, all the rats were anaesthetized intraperitoneally (I.P) and blood samples were collected by cardiac puncture for biochemical analysis. There was an increase in the BW of the control group and varying doses of tri-herbal formulation. It caused 88% decrease in FBSL; 371.7%, 386.6% and 296.0% with respect to 100, 200 and 400 mg/kg. Sub-chronic study of the effect of the extract showed a significant increase (P<0.05) in packed cell volume (PCV), white blood counts in rat induced diabetes. The histological studies showed that the diabetic rats with the architecture of the pancreas distorted, was restored to normal by the extract. Its LD50 was found to be greater than 1000 mg/kg indicating its safety in rats. This study has shown that triherbal formula has hypoglycemic and hematological effects.


Antidiabetic, Moringa oleifera, Ocimum gratissimum, Vernonia amygdalina, Alloxan-induced

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