Journal of Experimental Pathology

Original Research Open Access

Development of a Fast and Quantitative Method to Evaluate Oocytes in Experimental Rabbits

Yafei Zhang , Haoyun Li , Mengjun Dai , Minghui Feng , Yi Lou , Dandan Li , Ji Yang , Lingna Li , Juan Lv , Hui Zhang

The pathological evaluation of ovaries is a critical endpoint in reproductive toxicology assessments. Currently, there is no standardized method for the quantification of oocytes and follicles in preclinical drug safety evaluations. This study developed a simplified quantitative method based on stereological principles to efficiently assess the number of oocytes in experimental rabbits. The quantitative results obtained with this method are consistent with those from traditional counting methods.

J Exp Pathol, 2025, Volume 6, Issue 1, p1-7 | DOI: 10.33696/pathology.6.054

Commentary Open Access

Emerging Therapies for Congenital Melanocytic Nevi

Joshua Herron , Jessica L. Flesher , David E. Fisher

Congenital melanocytic nevi (CMN) are benign melanocytic neoplasms that vary in genetic drivers leading to the formation of pigmented lesions. Activation of Mitogen-Activated Protein Kinase (MAPK) pathway members through oncogenic mutations of NRAS or BRAF and more rarely by gene fusions allow expansion of nevomelanocytes to form CMN. Pre-clinical approaches to CMN therapy are expanding with the use of small-molecular inhibitors to mitogen-activated protein kinase kinase (MEK), RNA-based therapeutic approaches, and leveraging topical immunotherapy to regress nevi using in vitro and in vivo models and select case reports.

J Exp Pathol, 2025, Volume 6, Issue 1, p8-13 | DOI: 10.33696/pathology.6.055

Original Research Open Access

Comparison and Investigating the Effect of Using an Amniotic Membrane and Rectus Sheath to Repair Damage of the Tunica Albuginea in the Penis: An Animal Study on Rabbits

Mohammad Alishahi , Seyed Mokhtar Hosseini , Sina Ebrahimi , Anahita Rezaei , Mahboobeh Dehvari , Nima Bakhtiari , Negar Safarzadeh

In this study, the rectus sheath and amniotic membrane were utilized to repair tunica albuginea damage in rabbits. Ten male New Zealand white rabbits were included in the study. Group 1 consisted of 5 rabbits in which the tunica albuginea injury was repaired using amniotic membrane from pregnant rabbits. Group 2 consisted of 5 rabbits in which their rectus sheath was used to repair the tunica albuginea injury. Our results showed that both the use of amniotic membrane and rectus sheath are effective in repairing tunica albuginea damage in rabbits.

J Exp Pathol, 2025, Volume 6, Issue 1, p14-20 | DOI: 10.33696/pathology.6.056

Commentary Open Access

The Presence, Origins and Potential Role of Bi-hormonal Endocrine Cells Within the Pancreatic Islets of Langerhans

David J Hill

The pancreatic islets of Langerhans contain a minority of endocrine cells that contain more than one hormone: predominantly combinations of glucagon, insulin and somatostatin. A recent paper from our laboratory examined the ontogeny of such cells in the human pancreas and found that they persisted throughout the lifespan but altered in relative abundance with age. Glucagon/insulin bi-hormonal cell number significantly increased with age whilst insulin/somatostatin and glucagon/somatostatin cells significantly decreased.

J Exp Pathol, 2025, Volume 6, Issue 1, p21-28 | DOI: 10.33696/pathology.6.057