Research Article Open Access
Volume 1 | Issue 3 | DOI: https://doi.org/10.33696/haematology.1.014

Second Generation Platelet Concentrates - L-PRF (Fibrin Rich in Platelets and Leukocytes) and Its Derivatives (A-PRF, I-PRF)-: Morphological Characteristics to be Used in Modern Regenerative Surgery. Experimental Research

  • 1School of Medicine, University of Salerno Italy, 84084 Fisciano SA, Italy
  • 2Unit of Dermosurgery Cutaneous Transplantations and Hard-to-HealWound,“Villa Fiorita”Private Hospital, 81031 Aversa CE, Italy
  • 3Institute for the Studies and Care of Diabetics, Abetaia, 81020 Casagiove CE, Italy
  • 4Colonel Veterinarian, Director of the Veterinary Service, HQ Carabinieri Army, Roma, Italy
  • 5Veterinarian Freelancer, Caserta, Italy
  • 6Faculty of Medicine and Surgery, Vasile Goldis Western University of Arad, 310025 Arad, Romania
  • 7Veterinarian Freelancer, Sulmona (AQ), Italy
+ Affiliations - Affiliations

Corresponding Author

Alessandro Crisci, alcrisci@unisa.it

Received Date: October 14, 2020

Accepted Date: November 09, 2020


Introduction and objective: Growing multidisciplinary field of tissue engineering aims to regenerate, enhance, or replace predictably damaged or missing tissues. We carried out a prospective study with equine blood for the evaluation of specific scientific techniques of second-generation platelet concentrates in terms of Weight, Length, Width, Thickness, Surface in clots and membranes of L-PRF and A-PRF so that they are readily available and relatively easy to use in the daily clinical routine.

Methods: Horse blood, which was collected in anticoagulant-free PET tubes with silica for clot production and L-PRF membranes and in glass tubes for clots and A-PRF membranes in six healthy horses. Membranes and the clots produced were examined.

Results: Horse L-PRF/A-PRF membranes produced at the time Ø after centrifugation are made up of 66% red blood cells, 1.56% white blood cells and 32% platelets. Average morphological characteristics (± D.S.) detected among the various types of clots and membranes are: Weight of the clot gr. 3.52 (± 0.64); Exudate weight gr. 2.54 (± 0.48); Membrane weight gr. 0.84 (± 0.15); Coagulum length 40.8 mm (± 4.40); Clot width mm 13.89 (± 1.18); Coagulum thickness mm 6.48 (± 0.92); Coagulum surface mm2 48.64 (± 5.65); Membrane length 34.37 mm (± 3.79); Clot width mm 11.55 (± 1.58); Coagulum thickness mm 2.78 (± 0.38); Coagulum surface mm2 35.78 (± 5.67); Clot/Blood Sample weight ratio 10ml% 22.15 (± 2.20). Most useful values in clinical terms are those obtained in the A-PRF of the clot surface 53.94 (± 14.39) mm2 p=0.001 <0.05 and of the membrane surface 43.52 (± 8.68) mm2 p=0.560> 0.05.

Conclusion: Our studio has tried to standardize the L-PRF/A-PRF preparation procedure, which while remaining easy to perform and low-cost technique, does not require specialized equipment and has a certain consistency in the production of an L-PRF membrane/A-PRF in terms of macroscopic and microscopic characteristics.


Blood derivatives; Growth factors; Leucocyte and platelets-rich fibrin; Advanced platelets-rich fibrin; L-PRF wound box; Stem cells

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