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Journal of Clinical Haematology

ISSN: 2766-4686

Research Article Open Access
Volume 1 | Issue 3 | DOI: https://doi.org/10.33696/haematology.1.014

Second Generation Platelet Concentrates - L-PRF (Fibrin Rich in Platelets and Leukocytes) and Its Derivatives (A-PRF, I-PRF)-: Morphological Characteristics to be Used in Modern Regenerative Surgery. Experimental Research

Alessandro Crisci1,2,3,*, Carlo Alberto Minniti 4, Antonella Conte5, Michela Crisci6, Federica Cardillo7
  • 1School of Medicine, University of Salerno Italy, 84084 Fisciano SA, Italy
  • 2Unit of Dermosurgery Cutaneous Transplantations and Hard-to-HealWound,“Villa Fiorita”Private Hospital, 81031 Aversa CE, Italy
  • 3Institute for the Studies and Care of Diabetics, Abetaia, 81020 Casagiove CE, Italy
  • 4Colonel Veterinarian, Director of the Veterinary Service, HQ Carabinieri Army, Roma, Italy
  • 5Veterinarian Freelancer, Caserta, Italy
  • 6Faculty of Medicine and Surgery, Vasile Goldis Western University of Arad, 310025 Arad, Romania
  • 7Veterinarian Freelancer, Sulmona (AQ), Italy
+ Affiliations - Affiliations

Corresponding Author

Alessandro Crisci, alcrisci@unisa.it

Received Date: October 14, 2020

Accepted Date: November 09, 2020

Citation:

Crisci A, Minniti CA, Conte A, Crisci M, Cardillo F. Second Generation Platelet Concentrates - L-PRF (Fibrin Rich in Platelets and Leukocytes) and Its Derivatives (A-PRF, I-PRF)-: Morphological Characteristics to be Used in Modern Regenerative Surgery. Experimental Research. J Clin Haematol. 2020; 1(3):90-102.


Copyright: © 2020 Crisci A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Introduction and objective: Growing multidisciplinary field of tissue engineering aims to regenerate, enhance, or replace predictably damaged or missing tissues. We carried out a prospective study with equine blood for the evaluation of specific scientific techniques of second-generation platelet concentrates in terms of Weight, Length, Width, Thickness, Surface in clots and membranes of L-PRF and A-PRF so that they are readily available and relatively easy to use in the daily clinical routine.

Methods: Horse blood, which was collected in anticoagulant-free PET tubes with silica for clot production and L-PRF membranes and in glass tubes for clots and A-PRF membranes in six healthy horses. Membranes and the clots produced were examined.

Results: Horse L-PRF/A-PRF membranes produced at the time Ø after centrifugation are made up of 66% red blood cells, 1.56% white blood cells and 32% platelets. Average morphological characteristics (± D.S.) detected among the various types of clots and membranes are: Weight of the clot gr. 3.52 (± 0.64); Exudate weight gr. 2.54 (± 0.48); Membrane weight gr. 0.84 (± 0.15); Coagulum length 40.8 mm (± 4.40); Clot width mm 13.89 (± 1.18); Coagulum thickness mm 6.48 (± 0.92); Coagulum surface mm2 48.64 (± 5.65); Membrane length 34.37 mm (± 3.79); Clot width mm 11.55 (± 1.58); Coagulum thickness mm 2.78 (± 0.38); Coagulum surface mm2 35.78 (± 5.67); Clot/Blood Sample weight ratio 10ml% 22.15 (± 2.20). Most useful values in clinical terms are those obtained in the A-PRF of the clot surface 53.94 (± 14.39) mm2 p=0.001 <0.05 and of the membrane surface 43.52 (± 8.68) mm2 p=0.560> 0.05.

Conclusion: Our studio has tried to standardize the L-PRF/A-PRF preparation procedure, which while remaining easy to perform and low-cost technique, does not require specialized equipment and has a certain consistency in the production of an L-PRF membrane/A-PRF in terms of macroscopic and microscopic characteristics.

Keywords

Blood derivatives; Growth factors; Leucocyte and platelets-rich fibrin; Advanced platelets-rich fibrin; L-PRF wound box; Stem cells

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