Introduction and objective: Growing multidisciplinary field of tissue engineering aims to regenerate, enhance, or replace predictably damaged or missing tissues. We carried out a prospective study with equine blood for the evaluation of specific scientific techniques of second-generation platelet concentrates in terms of Weight, Length, Width, Thickness, Surface in clots and membranes of L-PRF and A-PRF so that they are readily available and relatively easy to use in the daily clinical routine.
Methods: Horse blood, which was collected in anticoagulant-free PET tubes with silica for clot production and L-PRF membranes and in glass tubes for clots and A-PRF membranes in six healthy horses. Membranes and the clots produced were examined.
Results: Horse L-PRF/A-PRF membranes produced at the time Ø after centrifugation are made up of 66% red blood cells, 1.56% white blood cells and 32% platelets. Average morphological characteristics (± D.S.) detected among the various types of clots and membranes are: Weight of the clot gr. 3.52 (± 0.64); Exudate weight gr. 2.54 (± 0.48); Membrane weight gr. 0.84 (± 0.15); Coagulum length 40.8 mm (± 4.40); Clot width mm 13.89 (± 1.18); Coagulum thickness mm 6.48 (± 0.92); Coagulum surface mm2 48.64 (± 5.65); Membrane length 34.37 mm (± 3.79); Clot width mm 11.55 (± 1.58); Coagulum thickness mm 2.78 (± 0.38); Coagulum surface mm2 35.78 (± 5.67); Clot/Blood Sample weight ratio 10ml% 22.15 (± 2.20). Most useful values in clinical terms are those obtained in the A-PRF of the clot surface 53.94 (± 14.39) mm2 p=0.001 <0.05 and of the membrane surface 43.52 (± 8.68) mm2 p=0.560> 0.05.
Conclusion: Our studio has tried to standardize the L-PRF/A-PRF preparation procedure, which while remaining easy to perform and low-cost technique, does not require specialized equipment and has a certain consistency in the production of an L-PRF membrane/A-PRF in terms of macroscopic and microscopic characteristics.
Blood derivatives; Growth factors; Leucocyte and platelets-rich fibrin; Advanced platelets-rich fibrin; L-PRF wound box; Stem cells