Background: ‘Seirogan’ is a well-known Japanese traditional herbal medicine for stomachache or diarrhea due to digestive disorders. Recently, it was reported that the administration of Seirogan alleviated gastric anisakiasis symptoms, and that Seirogan quickly suppressed the motility of Anisakis larvae in vitro.
Objectives: Objective is to determine whether the Seirogan-treated anisakid and raphidascaridid nematodes are just paralyzed or killed.
Methods: We tried to stain Seirogan-treated (1 pill/10 ml) nematodes with trypan blue stain which is widely used for the selective staining of dead tissues.
Results: Seirogan-treated anisakid and raphidascaridid nematodes were stained blue by trypan blue. In addition, Seirogan-treated nematodes were digested by pepsin treatment within 24 h. These results indicate that Seirogan kills anisakid and raphidascaridid nematodes, and suggest that Seirogan may be strongly effective for the treatment of gastrointestinal diseases caused by nematodes.
Conclusion: A widely available intestinal medicine ‘Seirogan’ kills anisakid and raphidascaridid nematodes at its normal dose, and the killed nematodes are soon digested by pepsin.
Anisakid nematodes, Seirogan; Nematocidal effect