Research Article Open Access
Volume 1 | Issue 1 | DOI: https://doi.org/10.33696/Pharmacol.1.005

Molecular Detection of Plasmid - Mediated Quinolone Resistant Genes in Uropathogenic E. coli from Tertiary Referral Hospital in Tehran , Iran

  • 4Department of Microbiology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  • 1Children’s Medical Center Hospital, Tehran University of Medical Sciences, Tehran, Iran
  • 2Department of Pediatric Infectious Disease, Research Center of Pediatric Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran
  • 3Department of bacteriology, Pasteur Institute of Iran, Tehran, Iran
+ Affiliations - Affiliations

Corresponding Author

Azardokht Tabatabaei, azardokht _tabatabaei@yahoo.com

Received Date: February 24, 2019

Accepted Date: August 13, 2019


Background: Fluoroquinolone antibiotics are usually used for the treatment of urinary tract infections. The aim of this study was to determine the prevalence and molecular characterization of Plasmid-Mediated Quinolone Resistance (PMQR) genes among ESBL-producing Escherichia coli isolates obtained from tertiary referral hospital in Tehran, Iran.

Methods: A total of 150 uropathogenic E. coli isolates were obtained from Rasool-e- Akram hospital. The bacterial isolates were identified by standard laboratory methods. Then, the susceptibility to quinolone antibiotics was assessed by standard disk diffusion method. The PCR method was used to show presence of qnrA, qnrB, qnrS, aac(6)-Ibcr and qepA genes.

Results: Overall, 79 of the 150 isolates (52.6%) were non-susceptible to quinolone antibiotics. Out of 79 quinolone non-susceptible isolates, 46 (58.2%) isolates harboured PMQR-encoding genes. Further, 36 (24%) had aac(6)-Ib-cr gene and interestingly, amplification assays showed that 33 (41.8%) out of 79 quinolone non-susceptible isolates carried only qnrB gene. Also qnrA, qnrB (30.9%), qepA (7.3%) and qnrS (25.4%) genes showed. ,

Conclusions: This study showed a high prevalence of aac(6)-Ib-cr, qnrB, qnrS and qnrA genes in the uropathogenic E. coli isolates from tertiary referral hospital. Therefore, the application of proper infection control and well-established antibiotic prescription guidelines seems to be highly needed in our medical centers.


Quinolones resistance; Urinary tract infection; aac(6)-Ib-cr, Pyelonephritis; Cystitis

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