Miscanthus capensis is a hardy, evergreen, medium-high, clump-forming grass that belongs to the Poaceae family. It is used in South Africa for the treatment of pimples, wounds, eczema, and acne. The present study was investigated to examine the biological activity of Miscanthus capensis roots extract. The effect of the plant extract on protein glycation and collagen production was investigated using in vitro assays. The plant demonstrated strong inhibition of protein glycation and also exhibited an increase in collagen production in human dermal fibroblast (MRHF) cells at all the concentrations tested. The result observed in this study further complements what was reported in our previous study with respect to the understudy plant in eradicating skin related diseases.