Commentary - Journal of Cellular Signaling (2021) Volume 2, Issue 2
Phosphorylation of RIAM Activates Its Adaptor Function in Mediating Integrin Signaling
Baihao Su, Jinhua Wu*
Molecular Therapeutics Program, Fox Chase Cancer Center, Philadelphia, PA 19111, USA
- *Corresponding Author:
- Jinhua Wu
Received date: April 28, 2021; Accepted date: May 27, 2021
Citation: Su B, Wu J. Phosphorylation of RIAM Activates Its Adaptor Function in Mediating Integrin Signaling. J Cell Signal
2021; 2(2): 103-110.
Copyright: © 2021 Su B, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Integrins are cellular receptors that regulate cell adhesion and many other cellular functions. Integrins can be activated via an “insideout
pathway” that is promoted by RAP1 GTPase. RAP1-GTP-Interacting Adaptor Molecular (RIAM) mediates integrin activation by
linking RAP1 GTPase to talin, an integrin activator. RIAM’s function in integrin signaling is tightly regulated. In this commentary, we
review recent studies of the molecular mechanisms underlying RIAM autoinhibition via both intramolecular interaction and oligomer
assembly, and the phosphorylation-dependent activation of RIAM.
RIAM, Integrin, Phosphorylation, RAP1, FAK, SRC, Lamellipodin
Extracellular matrix (ECM) surrounding cells in solid tissue provides mechanical support and communicates with cells through cell adhesion molecules (CAMs) at the cell surface [1,2]. Integrin is a major group of CAMs represented by a family of αβ heterodimeric single transmembrane receptors . At least 18 integrin α subunits and eight integrin β subunits have been identified. Particular pairs of α and β subunits generate 24 distinct integrin species in vertebrates . While some integrin species, such as β1 and αv integrins, are ubiquitously expressed, others are expressed in a tissue-specific manner. In particular, β2 integrins are expressed exclusively on lymphocytes, whereas αIIbβ3 is strictly expressed on platelets . Hence, abnormal expression and mis-regulated activity of integrins may lead to thrombotic disorders, cardiovascular diseases, T cell proliferation defects, and many autoimmune diseases [4,5]. Integrins are emerging as appealing targets for therapeutic intervention, primarily by antagonizing extracellular ligand binding . Indeed, two integrin inhibitors, Natalizumab and Vedolizumab, have been approved for treating inflammatory bowel diseases [6,7].
Conformational change of the ectodomains from a lowaffinity binding state to a high-affinity binding state is a hallmark of integrin activation [8-10]. Two 4.1-ezrinradixin- moesin (FERM) domain-containing proteins, talin and kindlin, govern this event in response to the activation of a small GTPase, Ras-associated protein 1 (RAP1) [11-13]. These two activators interact with the
cytoplasmic tails of the integrin β subunit, leading to
conformational change in the integrin ectodomains for
ligands binding . This signaling cascade from RAP1
to integrin is known as the “inside-out” integrin signaling
pathway. In lymphocytes, the interaction of talin and
integrin is promoted by a RAP1 effector known as Rap1-
GTP-Interacting Adaptor Molecule (RIAM) [2,15]. RIAM
is recruited to the plasma membrane (PM) by GTP-bound,
active RAP1, and subsequently recruits talin to the PM
where talin activates integrin. Upon activation, integrin
engages with the ECM ligands and modulates cytoskeleton
remodeling through an “outside-in” signaling pathway by
activating downstream signaling molecules such as Focal
adhesion kinase (FAK), integrin-linked kinase (ILK), and
Src, leading to a multitude of cellular processes including
cell migration, survival, and proliferation [16-19]. In this
commentary, we review recent findings regarding the
molecular mechanisms underlying RIAM autoinhibition
and activation via phosphorylation in the “inside-out”
RIAM Mediates Integrin Activation
RIAM is abundantly expressed in leukocytes and is
essential for the signaling of leukocyte-specific integrins.In particular, T cells highly express two types of β integrins
that include VLA-4 (α4β1), α5β1, and α6β1 of β1 integrins and LFA-1 (αLβ2) of β2 integrins . RIAM has been shown to regulate the activation of VLA-4 and LFA-1 [21,22],
the two integrins that play important roles in leukocyte
trafficking during immune response [23,24]. RIAM has
also been shown to regulate actin dynamics in T cells
through Enabled/Vasodilator-stimulated phosphoprotein
(Ena/VASP) family proteins and profilin . Indeed,
conventional T cells derived from RIAM knock-out mice
exhibit impaired adhesion and defect of lymphocytes
trafficking . It is worth noting that RIAM’s function in
regulatory T cells, when knocked out, can be compensated
by a RIAM paralogue in mammalian known as lamellipodin
(Lpd) , suggesting a rescue mechanism by regulating
the expression level of paralogues. Subcellular localization
of RIAM is dependent on the activation states of cells and
cell types. RIAM is concentrated at the PM and cytoskeleton
upon T cell activation [2,27]. RIAM is also recruited to the
tips of lamellipodia when overexpressed in B16-F1, Swiss
3T3, and HEK293 cells [25,28], and to both lamellipodial
tips and focal adhesions in stimulated NIH3T3 cells .
RIAM is a RAP1 effector and mediates the “inside-out” integrin activation by activating talin and recruiting it to the PM [29,30]. The central structural module of RIAM accommodates a Ras-association (RA) domain that binds to RAP1 and a Pleckstrin-homology (PH) domain that engages with the PM. The two domains tightly contact each other through a hydrophobic interface and form a stable “RA-PH” structure module that are also seen in the Grb family adapter proteins . At the amino side to the RA-PH, RIAM possesses a negative charged talinbinding segment (TBS), followed by an autoinhibitory segment (IN), two poly-proline (PP) motifs, and a coiledcoil (CC) region adjacent to the RA domain (Figure 1). The carboxyl-terminal side of RA-PH is largely disordered with
multiple poly-proline (PP) motifs for EVH1 domain and
profilin binding . Upon RAP1 activation, the RA-PH
module drives RIAM to the PM by associating with GTPbound
RAP1 via the RA domain, and with phosphoinositol
4,5-bisphosphate (PI(4,5)P2) in the PM via the PH domain
. Subsequently, RIAM recruits talin to the PM through
the TBS segment by interacting with talin in the rod region
. RIAM also activates talin by an interaction of TBS
with talin F3 domain in the head region. This interaction
occludes the association of the F3 domain with the
autoinhibitory domain in talin rod region, allowing the
talin head F3 domain to interact with integrin β subunit
Phosphorylation in Integrin Signaling
The structural properties and the resulting activity states of these signaling proteins are often controlled by various types of post-translational modifications (PTMs). Among all PTMs, phosphorylation is the most commonly observed event in the integrin signaling pathway. The earliest studies of phosphorylation events in the integrin signaling pathway can be traced back to the 1980s [34-36]. It has been shown that phosphorylation of talin and kindlin regulates their functions in mediating integrin activation, and may alter their binding preference to certain integrin species [37-39]. Among other kinases, FAK and Src family kinases have been well documented for their roles in integrin signaling pathway.
FAK was known for its function of regulating signaling downstream of integrin activation. Integrin-mediated cell adhesion to ECM relieves FAK from autoinhibition, allowing phosphorylation of its activation loop [40,41].
Activated FAK interacts with intracellular signaling molecules such as Src, PI3-K, Grb7, thereby regulating
various cellular functions including cell growth, cell
migration, and intracellular trafficking. Src kinases
have been shown to phosphorylate integrin and related
adaptor proteins. The Spleen tyrosine kinase (Syk), a Srcfamily
kinase, phosphorylates β3 integrin at the kindlinbinding
site . A lymphocyte-specific Src kinase, LCK,
phosphorylates Lymphocyte function-associated antigen 1
(LFA-1, αLβ2 integrin) and triggers downstream signaling that activates Phosphoinositide-3 kinase (PI3-K) and Syk . In addition, Src also phosphorylates kindlin-2,
vinculin, and tensin that play essential roles in promoting
integrin-mediated focal adhesion and cell spreading
[44-48]. Recently, both FAK and Src kinases have been
reported to regulate integrin signaling by activating
distinct functions of RIAM [49,50].
RIAM Autoinhibition is Relieved by FAK and Src Phosphorylation
RIAM adapts an auto-inhibition conformation in which the IN segment binds to the RA domain. The IN:RA interface involves multiple side chain interactions that include Tyr45, Glu60, and Asp63 in the IN segment, and is further stabilized by the CC segment that sits on top of the IN segment . Both Glu60 and Asp63 form salt bridges with Lys213 in the α1 helix of the RIAM RA domain. In the RAP1: RIAM complex structure, Lys213 of RIAM forms a signature interaction with Asp33 of RAP1, and a K213A mutation abolished the RAP1:RIAM interaction . Thus, the IN segment masks the RAP1-binding site in the RA domain, resulting in autoinhibition of the RIAM RA domain. Releasing of this autoinhibition of RIAM for RAP1 binding requires phosphorylation at Tyr45. The bulky and negatively charged phosphoryl group of the pTyr45 disrupts the compact autoinhibitory interface, thus exposing the RA domain for RAP1 binding. Indeed, the phosphomimetic mutant Y45E or an interface mutation E60A/D63A enhance the in vitro RAP1:RIAM interaction and the PM co-localization of RAP1 and RIAM. Bioinformatic analyses suggest that Tyr45 is a substrate of FAK kinase, which was further confirmed by phosphorylation analyses using cells treated with FAK-specific inhibitors . It is noteworthy that FAK is well known for its function in mediating “outside-in” integrin signaling at focal adhesions upon binding of integrins to the extracellular matrix. This new finding of FAK-dependent RIAM activation, therefore, reveals a crosstalk between “outside-in” and “insideout” integrin signaling, by which integrin activation is amplified through a feedforward mechanism. In addition to Tyr45, endogenous phosphorylation of Ser55 in the IN segment has also been reported . We have shown that RIAM bearing a phosphomimetic mutant of Ser55 also escapes from RA domain autoinhibition. Nevertheless, no notable change in the phosphorylation levels of Ser55 in anti-CD3 antibody stimulated Jurkat T cells was detected by proteomic analysis, suggesting that RIAM activation via Ser55 phosphorylation may be independent of T cell activation .
Besides the RA domain, the RAP1-dependent RIAM translocation to the PM also requires the PH domain to engage PI(4,5)P2 in the PM . While the RA domain is autoinhibited via an intra-molecular interaction prior to RIAM activation, the PH domain is also autoinhibited via an inter-molecular interaction that blocks its PI(4,5) P2-binding site. This autoinhibitory interaction of the PH domain was first revealed by structural analyses and further validated by biochemical and functional characterization. Five crystal structures that contain the RA-PH module of RIAM have been reported . These include a RA-PH alone structure (PDB: 6OLU), two structures of RA-PH with the CC segment (cc-RA-PH) in different crystal forms (PDB: 3TCA and 6O6H), a RA-PH:RAP1 complex structure (PDB: 4KVG), and an intramolecularly autoinhibited structure (IN-CC-RA-PH, PDB: 6E31) [2,49,50,53]. These structures were analyzed for conserved crystal packing interfaces to identify putative dimer assembly. While two of the five structures (PDBs: 3TCA and 4KVG) possess a common pseudo-symmetric intermolecular interface, all five structures share a conserved asymmetrical interface. It has long been suspected that RIAM adopts a dimer (or higher oligomer) state as found in its signaling partner talin and its mammalian paralogue Lpd [54,55]. These two conserved crystal contacts, both mediated by the PH domain, may represent dimer configurations of RIAM. We assessed RIAM oligomerization in a coimmunoprecipitation (co-IP) assay using full-length RIAM with two different tags (HA and GFP). Although the pseudo-symmetric interface is significantly larger than the asymmetrical interface, no notable effect in the oligomerization state of RIAM was observed in the pseudo-symmetric interface mutations (H389A/Y398A), suggesting that this interface is not physiologically relevant but is only a result of crystal packing. In contrast, RIAM A435Y mutation that disturbs the asymmetrical interface exhibited reduced dimerization in the co-IP assay, suggesting that the asymmetrical interface formed by the PH domain helical extension from one molecule and two β strains of another PH domain mediates RIAM dimerization . Interestingly, the helical extension is only seen in the PH domain of RA-PH-containing proteins such as Grb7/10/14, and has also been shown to mediate the dimerization of Grb10 . RIAM dimerization was further validated by size exclusion chromatography (SEC) analyses. Nevertheless, our unpublished work indicated no dimer formation of recombinant RA-PH alone in solution, suggesting that other regions of RIAM are also required to stabilize the dimer configuration of RA-PH.
Masking of the PI(4,5)P2-binding site in the dimer configuration of RIAM RA-PH results in the suppression of the PM translocation of RIAM. The two β strains of the neighboring PH domain that interact with the helical extension accommodate a positively charged patch composed of Lys327, Lys328, Lys331, Arg332, and Arg333 that engages with PI(4,5)P2 in the PM in response to RAP1 activation . This PI(4,5)P2-binding site needs to be unmasked for RIAM translocation to the PM. Indeed, RIAM-A435Y, the helical extension mutation that disrupts dimer assembly and unmasks the PI(4,5)P2-binding site, exhibited enhanced PM translocation . Consequently, RIAM-A435Y up-regulates integrin activity in CHO-A5 cells that stably express αIIbβ3 integrin. Similar to the RA domain autoinhibition, this dimerization-induced inhibition of PH: PI(4,5)P2 interaction is also regulated by phosphorylation. RIAM dimerization is significantly reduced upon phosphorylation as indicated by the co-IP assay. Moreover, RIAM is highly phosphorylated during T cell activation, and the phosphorylation of RIAM is coupled with its PM translocation, consistent with the model that phosphorylation of RIAM disrupts RIAM dimerization, in turn promotes its association with the PM. Further analyses revealed that RA-PH module is the substrate of Src family kinases. An LCK inhibitor, but not FAK inhibitors, suppressed the phosphorylation of the RA-PH module of RIAM upon T cell activation . The LCK inhibitor (RK- 24466) also suppressed the PM translocation of RIAM in Jurkat T cells and LFA-1-mediated T cell adhesion . Mass spectrometry analyses revealed four tyrosines that are highly phosphorylated by LCK and Fyn, two Src family kinases abundant in lymphocytes. Among the four tyrosines, Tyr267 and Tyr427 reside near the dimer interface, and Tyr398 is located in the non-physiological, crystal packing interface, whereas Tyr277 is in a disordered loop. Therefore, phosphomimetic mutations of Tyr267 and Tyr427 (Y267E, Y427E, or Y427E/Y427E) were expected to diminish the dimer assembly via the PH domain. Indeed, these mutations promoted RIAM PM localization in both Jurkat T cells and HEK293T cells. In contrast, the nonphosphorylable mutations, Y267F or Y427F, severely suppressed RAP1-mediated RIAM PM localization as the RA-PH module is locked in the inhibitory dimer assembly. Together, these results indicated that the phosphorylation of Tyr267 and Tyr427 in RIAM RA-PH by Src family kinases unmasks the PI(4,5)P2-binding site in RIAM PH domain, thus promoting the PM translocation of RIAM and subsequent integrin activation .
To our knowledge, this is the first example of such a regulatory mechanism of a PH domain, in which the exposure of PI(4,5)P2-binding site in the PH domain is regulated by homo-oligomerization and phosphorylation. Nevertheless, bioinformatic analyses of crystal structures of PH domains using ProtCID revealed that Lpd may also possess a similar oligomer assembly that masks the PI(4,5)P2 binding site . The RA-PH module in Lpd can be phosphorylated by Abl and Src kinases. In vitro kinase assays using synthetic peptides derived from Lpd suggested six tyrosines in the PH domain that were phosphorylated by both Abl and Src [57,58]. Structural alignment of the
PH domains of Lpd and RIAM reveals that four of the six
sites correspond to the phosphorylation sites in the RIAM
PH domain including Lpd-Tyr357 (RIAM-Tyr267), Lpd-
Tyr366 (RIAM-Tyr277), Lpd-Tyr481 (RIAM-Tyr398),
and Lpd-Tyr510 (RIAM-Tyr427) [2,54], supporting our
speculation that the PI(4,5)P2-binding site in Lpd may
be regulated by a similar, phosphorylation-dependent
mechanism. Moreover, Lpd’s function in cell motility is
regulated by phosphorylation mediated by Abl and Src.
Lpd interacts with both the EVH1 domain of Ena/VASP
and Abi of the SCAR/WAVE complex via the C-terminal
polyproline motifs. Although the binding specificity to
EVH1 or Abi of these polyproline motifs remains unclear,
the interaction of Lpd with Ena/VASP or with SCAR/
WAVE complex is controlled by Lpd phosphorylation.
Phosphorylation of Lpd by Src only promotes its association
with the SCAR/WAVE complex, while phosphorylation of
Lpd by Abl promotes the interaction of Lpd and Ena/VASP
and its association with the SCAR/WAVE complex [57,58]. The in vitro kinase assays suggest similar phosphorylation patterns of Lpd in the C-terminal region by Abl and Src kinases [57,58]. In cells, however, phosphorylation
levels of these sites in the context of the full-length Lpd
by Abl and Src may be significantly different due to local
structural environment of the substrate motifs and cellular
or subcellular abundance of the kinases. Thus, analyses
of cell-based phosphorylation of properly folded Lpd are
needed to elucidate the molecular mechanisms underlying
this effector binding selectivity.
Other Forms of Regulation in Integrin Signaling
Together, these recent works have shown that both the RA and PH domains of RIAM are autoinhibited for the adaptor function in mediating integrin activity, and phosphorylation of RIAM mediated by FAK and Src kinases regulates RIAM activity (Figure 2). Other adaptors, such as kindlin and talin, have also been shown to promote integrin signaling upon phosphorylation [37,39]. Moreover, other types of PTMs were also found in integrin and related adaptors to regulate their cellular functions. Lysine acetylation in β1 integrin regulates fibronectin matrix assembly . Ubiquitination of the α5 integrin regulates the subcellular trafficking of α5β1 integrin . Proteolytic cleavage of talin and kindlin by calpain also regulates integrin-mediated cell adhesion [61,62]. In addition, vinculin has been shown to be
acylated by both myristic acid and the palmitic acid with unknown functions [63,64]. These PTMs may regulate
the functions of the adaptors by altering their structural
configurations, binding properties, and homo-/heterooligomer
In addition to PTMs, the function of the adaptors can also be modified prior to translation by RNA splicing, which often results in the loss of distinct functional modules in various isoforms. For instance, RIAM has a splicing isoform that lacks the PH domain and the C-terminal polyproline motifs , and Lpd possesses at least nine splicing isoforms, many lacking the entire C-terminal polyproline region . One can speculate that the function of these shorter isoforms in regulating cytoskeletal rearrangement may be largely compromised. It is therefore imperative to devote more efforts on the understanding of the regulatory mechanisms driven by these PTMs and various isoforms, particularly in the signaling pathways mediated by integrins or small GTPases.
This work is supported by an NIH grant GM119560 and a
Pennsylvania Department of Health Grant 6914701.
- Anthis NJ, Campbell ID. The tail of integrin activation.
Trends Biochem Sci. 2011;36(4):191-8.
- Wynne JP, Wu J, Su W, Mor A, Patsoukis N, Boussiotis
VA, et al. Rap1-interacting adapter molecule (RIAM)
associates with the plasma membrane via a proximity
detector. J Cell Biol. 2012;199(2):317-30.
- Takada Y, Ye X, Simon S. The integrins. Genome Biol.
- Huveneers S, Truong H, Danen HJ. Integrins: signaling,
disease, and therapy. Int J Radiat Biol. 2007;83(11-
- Desgrosellier JS, Cheresh DA. Integrins in cancer:
biological implications and therapeutic opportunities. Nat
Rev Cancer. 2010;10(1):9-22.
- Soler D, Chapman T, Yang LL, Wyant T, Egan R, Fedyk
ER. The binding specificity and selective antagonism of
vedolizumab, an anti-alpha4beta7 integrin therapeutic
antibody in development for inflammatory bowel diseases.
J Pharmacol Exp Ther. 2009;330(3):864-75.
- Selewski DT, Shah GV, Segal BM, Rajdev PA, Mukherji
SK. Natalizumab (Tysabri). AJNR Am J Neuroradiol.
- Coller BS. Activation affects access to the platelet
receptor for adhesive glycoproteins. J Cell Biol.
- O’Toole TE, Loftus JC, Du XP, Glass AA, Ruggeri ZM,
Shattil SJ, et al. Affinity modulation of the alpha IIb beta 3
integrin (platelet GPIIb-IIIa) is an intrinsic property of the
receptor. Cell Regul. 1990;1(12):883-93.
- Sims PJ, Ginsberg MH, Plow EF, Shattil SJ. Effect
of platelet activation on the conformation of the plasma
membrane glycoprotein IIb-IIIa complex. J Biol Chem.
- Li H, Deng Y, Sun K, Yang H, Liu J, Wang M, et al.
Structural basis of kindlin-mediated integrin recognition
and activation. Proc Natl Acad Sci U S A. 2017;114(35):9349-
- Zhang P, Azizi L, Kukkurainen S, Gao T, Baikoghli M,
Jacquier MC, et al. Crystal structure of the FERM-folded
talin head reveals the determinants for integrin binding.
Proc Natl Acad Sci U S A. 2020;117(51):32402-12.
- Zhang Y, Wang H. Integrin signalling and function in
immune cells. Immunology. 2012;135(4):268-75.
- Calderwood DA. Integrin activation. J Cell Sci.
- Lee HS, Lim CJ, Puzon-McLaughlin W, Shattil SJ,
Ginsberg MH. RIAM activates integrins by linking talin to
ras GTPase membrane-targeting sequences. J Biol Chem.
- Arias-Salgado EG, Lizano S, Sarkar S, Brugge JS, Ginsberg MH, Shattil SJ. Src kinase activation by direct
interaction with the integrin beta cytoplasmic domain.
Proc Natl Acad Sci U S A. 2003;100(23):13298-302.
- Huang MM, Lipfert L, Cunningham M, Brugge JS,
Ginsberg MH, Shattil SJ. Adhesive ligand binding to integrin
alpha IIb beta 3 stimulates tyrosine phosphorylation
of novel protein substrates before phosphorylation of
pp125FAK. J Cell Biol. 1993;122(2):473-83.
- Menter DG, DuBois RN. Prostaglandins in Cancer Cell
Adhesion, Migration, and Invasion. International Journal
of Cell Biology. 2012;2012:723419.
- Legate KR, Wickstrom SA, Fassler R. Genetic and cell
biological analysis of integrin outside-in signaling. Genes
- Hogg N, Laschinger M, Giles K, McDowall A. T-cell
integrins: more than just sticking points. J Cell Sci.
- Su W, Wynne J, Pinheiro EM, Strazza M, Mor A,
Montenont E, et al. Rap1 and its effector RIAM are required
for lymphocyte trafficking. Blood. 2015;126(25):2695-703.
- Walling BL, Kim M. LFA-1 in T Cell Migration and
Differentiation. Front Immunol. 2018;9:952.
- Yang GX, Hagmann WK. VLA-4 antagonists: potent
inhibitors of lymphocyte migration. Med Res Rev.
- Gaud G, Lesourne R, Love PE. Regulatory mechanisms
in T cell receptor signalling. Nat Rev Immunol.
- Lafuente EM, van Puijenbroek AA, Krause M,
Carman CV, Freeman GJ, Berezovskaya A, et al. RIAM,
an Ena/VASP and Profilin ligand, interacts with Rap1-
GTP and mediates Rap1-induced adhesion. Dev Cell.
- Sun H, Lagarrigue F, Wang H, Fan Z, Lopez-Ramirez
MA, Chang JT, et al. Distinct integrin activation pathways
for effector and regulatory T cell trafficking and function. J
Exp Med. 2021;218(2).
- Patsoukis N, Lafuente EM, Meraner P, Kim J,
Dombkowski D, Li L, et al. RIAM regulates the cytoskeletal
distribution and activation of PLC-gamma1 in T cells. Sci
- Jenzora A, Behrendt B, Small JV, Wehland J, Stradal
TE. PREL1 provides a link from Ras signalling to the
actin cytoskeleton via Ena/VASP proteins. FEBS Lett.
- Katagiri K, Hattori M, Minato N, Irie S, Takatsu K,
Kinashi T. Rap1 is a potent activation signal for leukocyte
function-associated antigen 1 distinct from protein kinase
C and phosphatidylinositol-3-OH kinase. Mol Cell Biol.
- Katagiri K, Hattori M, Minato N, Kinashi T. Rap1
functions as a key regulator of T-cell and antigen-presenting
cell interactions and modulates T-cell responses. Mol Cell
- Holt LJ, Daly RJ. Adapter protein connections:
the MRL and Grb7 protein families. Growth Factors.
- Chang YC, Zhang H, Franco-Barraza J, Brennan ML,
Patel T, Cukierman E, et al. Structural and mechanistic
insights into the recruitment of talin by RIAM in integrin
signaling. Structure. 2014;22(12):1810-20.
- Yang J, Zhu L, Zhang H, Hirbawi J, Fukuda K, Dwivedi
P, et al. Conformational activation of talin by RIAM
triggers integrin-mediated cell adhesion. Nat Commun.
- Hunter T, Sefton BM, Beemon K. Studies on the
structure and function of the avian sarcoma virus
transforming-gene product. Cold Spring Harb Symp
Quant Biol. 1980;44 Pt 2:931-41.
- David-Pfeuty T, Singer SJ. Altered distributions of
the cytoskeletal proteins vinculin and alpha-actinin in
cultured fibroblasts transformed by Rous sarcoma virus.
Proc Natl Acad Sci U S A. 1980;77(11):6687-91.
- Maher PA, Pasquale EB, Wang JY, Singer SJ.
Phosphotyrosine-containing proteins are concentrated in
focal adhesions and intercellular junctions in normal cells.
Proc Natl Acad Sci U S A. 1985;82(19):6576-80.
- Bialkowska K, Sossey-Alaoui K, Pluskota E, Izem L,
Qin J, Plow EF. Site-specific phosphorylation regulates the
functions of kindlin-3 in a variety of cells. Life Sci Alliance.
- Li Y, Luo X, Sun Y, Cui Z, Liu Y, Liu R, et al. High
Stoichiometry Phosphorylation of Talin at T144/T150
or S446 Produces Contrasting Effects on Calpainmediated
Talin Cleavage and Cell Migration. J Cancer.
- Jin JK, Tien PC, Cheng CJ, Song JH, Huang C, Lin
SH, et al. Talin1 phosphorylation activates beta1 integrins:
a novel mechanism to promote prostate cancer bone
metastasis. Oncogene. 2015;34(14):1811-21.
- Parsons JT. Focal adhesion kinase: the first ten years. J Cell Sci. 2003;116(Pt 8):1409-16.
- Hanks SK, Ryzhova L, Shin NY, Brabek J. Focal
adhesion kinase signaling activities and their implications
in the control of cell survival and motility. Front Biosci.
- Law DA, Nannizzi-Alaimo L, Phillips DR. Outside-in
integrin signal transduction. Alpha IIb beta 3-(GP IIb IIIa)
tyrosine phosphorylation induced by platelet aggregation.
J Biol Chem. 1996;271(18):10811-5.
- Gupta R, Palchaudhuri S, Chattopadhyay D.
Glutamate induces neutrophil cell migration by activating
class I metabotropic glutamate receptors. Amino Acids.
- Shi X, Yang J, Cui X, Huang J, Long Z, Zhou Y, et al.
Functional Effect of the Mutations Similar to the Cleavage
during Platelet Activation at Integrin beta3 Cytoplasmic
Tail when Expressed in Mouse Platelets. PLoS One.
- Sefton BM, Hunter T, Ball EH, Singer SJ. Vinculin:
a cytoskeletal target of the transforming protein of Rous
sarcoma virus. Cell. 1981;24(1):165-74.
- Zhang Z, Izaguirre G, Lin SY, Lee HY, Schaefer E,
Haimovich B. The phosphorylation of vinculin on tyrosine
residues 100 and 1065, mediated by SRC kinases, affects
cell spreading. Mol Biol Cell. 2004;15(9):4234-47.
- Ferrando IM, Chaerkady R, Zhong J, Molina H, Jacob
HK, Herbst-Robinson K, et al. Identification of targets of
c-Src tyrosine kinase by chemical complementation and
phosphoproteomics. Mol Cell Proteomics. 2012;11(8):355-69.
- Liu Z, Lu D, Wang X, Wan J, Liu C, Zhang H. Kindlin-2
phosphorylation by Src at Y193 enhances Src activity and
is involved in Migfilin recruitment to the focal adhesions.
FEBS Lett. 2015;589(15):2001-10.
- Cho EA, Zhang P, Kumar V, Kavalchuk M, Zhang H,
Huang Q, et al. Phosphorylation of RIAM by Src Promotes
Integrin Activation by Unmasking the PH Domain of
RIAM. Structure. 2020.
- Chang YC, Su W, Cho EA, Zhang H, Huang Q, Philips
MR, et al. Molecular basis for autoinhibition of RIAM
regulated by FAK in integrin activation. Proc Natl Acad Sci
U S A. 2019;116(9):3524-9.
- Zhang H, Chang YC, Brennan ML, Wu J. The
structure of Rap1 in complex with RIAM reveals specificity
determinants and recruitment mechanism. J Mol Cell
- Hornbeck PV, Zhang B, Murray B, Kornhauser
JM, Latham V, Skrzypek E. PhosphoSitePlus, 2014:
mutations, PTMs and recalibrations. Nucleic Acids Res.
- Zhang H, Chang YC, Huang Q, Brennan ML,
Wu J. Structural and Functional Analysis of a Talin
Triple-Domain Module Suggests an Alternative Talin
Autoinhibitory Configuration. Structure. 2016;24(5):721-
- Chang YC, Zhang H, Brennan ML, Wu J. Crystal
structure of Lamellipodin implicates diverse functions
in actin polymerization and Ras signaling. Protein Cell.
- Molony L, McCaslin D, Abernethy J, Paschal B,
Burridge K. Properties of talin from chicken gizzard
smooth muscle. J Biol Chem. 1987;262(16):7790-5.
- Depetris RS, Wu J, Hubbard SR. Structural and
functional studies of the Ras-associating and pleckstrinhomology
domains of Grb10 and Grb14. Nat Struct Mol
- Michael M, Vehlow A, Navarro C, Krause M. c-Abl,
Lamellipodin, and Ena/VASP proteins cooperate in dorsal
ruffling of fibroblasts and axonal morphogenesis. Curr
- Carmona G, Perera U, Gillett C, Naba A, Law AL,
Sharma VP, et al. Lamellipodin promotes invasive 3D
cancer cell migration via regulated interactions with Ena/VASP and SCAR/WAVE. Oncogene. 2016;35(39):5155-69.
- Vega ME, Kastberger B, Wehrle-Haller B,
Schwarzbauer JE. Stimulation of Fibronectin Matrix
Assembly by Lysine Acetylation. Cells. 2020;9(3).
- Lobert VH, Brech A, Pedersen NM, Wesche J, Oppelt
A, Malerod L, et al. Ubiquitination of alpha 5 beta 1
integrin controls fibroblast migration through lysosomal
degradation of fibronectin-integrin complexes. Dev Cell.
- Bate N, Gingras AR, Bachir A, Horwitz R, Ye F, Patel
B, et al. Talin contains a C-terminal calpain2 cleavage
site important in focal adhesion dynamics. PLoS One.
- Zhao Y, Malinin NL, Meller J, Ma Y, West XZ,
Bledzka K, et al. Regulation of cell adhesion and
migration by Kindlin-3 cleavage by calpain. J Biol Chem.
- Kellie S, Wigglesworth NM. The cytoskeletal
protein vinculin is acylated by myristic acid. FEBS Lett.
- Burn P, Burger MM. The cytoskeletal protein vinculin
contains transformation-sensitive, covalently bound lipid.
- UniProt C. UniProt: the universal protein
knowledgebase in 2021. Nucleic Acids Res.