Mini Review Open Access
Volume 2 | Issue 6 | DOI: https://doi.org/10.33696/immunology.2.056

Single-cell Approach to Generate Functional TCR-Ts: A Potential Accelerator of TCR-T Cell Therapy for Infectious Diseases

  • 1BGI-Shenzhen, Shenzhen 518103, China
  • 2China National GeneBank, BGI-Shenzhen, Shenzhen 518120, China
  • 3BGI Education Center, University of Chinese Academy of Sciences, Shenzhen 518083, China
  • 4Department of Biomedicine, Aarhus University, 8000, Aarhus, Denmark
  • 5School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China
  • 6Shenzhen Bay Laboratory, Shenzhen 518107, China
  • #These authors contributed equally
+ Affiliations - Affiliations

Corresponding Author

Linnan Zhu, zhuln@szbl.ac.cn

Received Date: July 23, 2020

Accepted Date: September 10, 2020


T cell-mediated immune response is essential for host defense against viruses, including the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) which has caused a global pandemic. Genetically engineered T cells with antigen-specific T cell receptors (TCR-Ts) are redirected to eliminate target cells via TCRs recognizing peptides bound with major histocompatibility complexes (MHCs). TCR-T cell therapy has proved effective in human trials involving solid tumors, while it is considered promising treatment for infectious diseases. To expedite the identification of functional TCRs and development of TCR-T cell therapy, we established an experimental pipeline to generate functional virus-specific TCR-Ts through coupled analysis of single-cell transcriptomic data and single-cell full length TCR V(D)J sequences. We validated this approach in selecting functional TCRs specific to a cytomegalovirus (CMV) pp65 epitope, NLV495-503, and published the results recently. In this commentary, we summarized the single-cell work flow and discussed its potential application in developing TCR-T cell therapy against SARS-CoV-2.


TCR-T, Single-cell RNA sequencing, Single-cell TCR V(D)J sequencing, SARS-CoV-2

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