Short Communication Open Access
Volume 3 | Issue 1 | DOI: https://doi.org/10.33696/AIDS.3.014

HIV RNA Load and Antiretroviral Drug Resistance of HIV-1 Strains in Chad on Dried Blood Spots: A Pilot Study

  • 1Service de Maladies infectieuses, Hôpital Le Bon Samaritain, CHU Walia, N’Djamena, Chad
  • 2Institut National de Recherche en Santé Publique, Bamako, Mali
  • 3EA 3064, Faculté de Médecine, Université Jean Monnet, Saint-Etienne, France
  • 4Laboratoire de Virologie médicale et moléculaire, Hôpital Robert Debré CHU Reims & EA-4684, Faculté de Médecine, Université Reims Champagne Ardennes, Reims, France
+ Affiliations - Affiliations

Corresponding Author

Laurent Andreoletti,  landreoletti@chu-reims.fr

Received Date: February 02, 2021

Accepted Date: March 15, 2021


We performed viral load (VL) and antiretroviral (ARV) drug resistance genotyping assays using dried blood spot samples (DBS) taken from HIV-1 infected patients in Chad. Among 80 adult patients naïve of any antiretroviral treatment regimen, 67 (84%) had a measured VL upper than 40 copies/ml (median; SD: 36891 copies/ml; [603-2 798 968]) of blood. Of them only 24 (36%) demonstrating a viral load upper was than 3.63 log10 copies/ml were successfully sequenced for performing a drug resistance genotyping assay. Of the 24 patients whom DBS samples were successfully sequenced, seven (29%) displayed the presence of an HIV-1 strain with known mutations conferring resistance against at least one ARV family. Our pilot study provides major data on the high prevalence levels of primary resistance to available ARV drugs in HIV-1 infected patients in Chad and highlights the need for a wide and rational use of HIV anti-integrase drugs in Sahelian area.


DBS, HIV viral load, Chad, NNRTI- or NRTI- primary resistant HIV strains

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